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Protein Science (1)

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Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein
STEPHEN J. BLANCE, NICOLA L. WILLIAMS, ZOË A. PRESTON, JOHN BISHARA, MICHAEL S. SMYTH, ANTHONY MAXWELL
Journal:

Protein Science / Volume 9 / Issue 5 / May 2000

Published online by Cambridge University Press:

01 May 2000, pp. 1035-1037

Print publication:

May 2000
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Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.