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heat capacity change

Protein Science (1)

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Heat capacity change for ribonuclease A folding
C. NICK PACE, GERALD R. GRIMSLEY, SUSAN T. THOMAS, GEORGE I. MAKHATADZE
Journal:

Protein Science / Volume 8 / Issue 7 / July 1999

Published online by Cambridge University Press:

01 July 1999, pp. 1500-1504

Print publication:

July 1999
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The change in heat capacity ΔCp for the folding of ribonuclease A was determined using differential scanning calorimetry and thermal denaturation curves. The methods gave equivalent results, ΔCp = 1.15 ± 0.08 kcal mol−1 K−1. Estimates of the conformational stability of ribonuclease A based on these results from thermal unfolding are in good agreement with estimates from urea unfolding analyzed using the linear extrapolation method.