The effects of sublethal concentrations of the insect growth regulators (IGRs) diflubenzuron and methoprene on some fitness components of an Aedes aegypti (L.) population in south-eastern Brazil were investigated. The fourth instar larvae of the mosquito were exposed to 20 ppb methoprene and 3 ppb diflubenzuron, and adult survivors were evaluated for fitness parameters, including body size and biomass, fecundity, fertility, longevity and symmetry. The sublethal concentrations had negative effects on longevity, but biomass, fecundity and fertility were not affected by either IGR. In separate experiments, A. aegypti individuals' surviving concentrations of 2, 3 and 3.5 ppb diflubenzuron and 5, 10 and 20 ppb methoprene were used for morphological analyses (size and symmetry). Diflubenzuron had negative effects on the mosquito's body symmetry. The observed decreases in longevity and symmetry may have negative impacts on the population dynamics of A. aegypti.

Des expérimentations de terrain en parcelles limitées ont permis, à partir de piégeages d'adultes, d'évaluer l'impactde huit insecticides sur des populations larvaires composées de 82 % de Chironomus salinarius, de 12 % de Chironomushalophilus et de Chironomus plumosus et 6 % d'autres espèces non identifées. Le diflubenzuron et les toxines de Bacillus thuringiensis H 14 et Bacillus sphaericus n'ont pas d'effet marqué aux doses détruisant les larves de moustiques. A ces mêmes doses, le fénitrothion, le téméphos et la deltaméthrine limitent fortement l'émergence. Le chlorpyrifos et le S 31183 (juvénoïde Sumitomo) sont très toxiques.

Dosage–mortality responses of F1 progeny of treated rice weevils, Sitophilus oryzae, indicated that the weevil is highly tolerant to R-20458. However, the ability of the insect growth regulator (IGR) to suppress F2 progeny production, demonstrated that R-20458 could be used as a protectant for grains.

The use of surfactants and their blends to improve the penetration of two insect growth regulators (IGRs; hydroprene and R-20458) into wheat kernels was investigated. It was found that formulation of IGRs with a surfactant, Tween-85 (polyoxyethylene trioleate), increased mortality and/or effectiveness of the IGRs. Formulation of hydroprene with a blend of Span-80 (sorbitan monooleate) with Tween-80 (polyoxyethylene monooleate) produced results comparable to those by hydroprene with Tween-85. However, formulations of IGRs with surfactants with high or low hydrophile-lipophile balance (HLB), failed to provide suitable control of the weevil, probably due to the type of emulsions formed. The same results were obtained with hydroprene formulations with blends of Spans and Tweens, even though their HLBs were close to that of Tween-85. Residual half-life for hydroprene was about three months; formulations with Tween-85 or a blend of Span-80 did not enhance the residual activity for hydroprene in wheat.

The insect growth regulator, 6′,7′-epoxy-3′-ethyl-7′-methyl-dec-2′-enyl 6-ethyl-3-pyridyl ether, at 10 ppm applied to rearing media, did not prevent oviposition or hatchability of the eggs of Tribolium confusum. The insect growth regulator (IGR) apparently had no effects on the development of younger larvae, and no morphological abnormalities were observed in these instars. However, the IGR prevented metamorphosis of the last larval instar. These larvae continued to moult several times, became larger than larvae in untreated food, but failed to pupate; as a result, no adults emerged from the treatments.

Late third stage (7-day-old) larvae and 1-day-old pupae of Anastrepha suspensa Loew (Diptera: Tephritidae) were dipped for 5 min in suspensions of 25% WP Dimilin® (diflubenzuron) at six dosages ranging from 0.003–0.1% a.i. Diflubenzuron caused significant (P < 0.05) mortality during the pupal stages of both groups treated. Consequently, the resulting adult populations were drastically reduced. The incidence of crumpled wings, deformed abdomens and ovipositors in these adults was 2–7 times and 4–9 times higher than the respective controls of treated larvae and pupae. The morphological abnormalities altered mating competitiveness of males and successful copulation and oviposition by females. Fecundity of females was reduced and diflubenzuron effects persisted into the first generation and caused decreased egg viability.

Adults fed a 0.1% a.i. diflubenzuron-treated diet for 27 days had no significant mortality, but had decreased fecundity. However, their pattern of oviposition did not differ from the control. Fecundity levels similar to the control's were achieved 6 days after the treated diet was replaced by normal diet. Diflubenzuron treatment of adults also drastically decreased the survivorship of first-generation individuals between egg-hatch and pupation. However, almost all individuals that pupated emerged as adults. No abnormalities were observed in first-generation adults or in the survivorship of their offspring.

Larvae of the Caribbean fruit fly, Anastrepha suspensa Loew (Diptera: Tephritidae) parasitized by the wasp, Biosteres longicaudatus Ashmead (Hymenoptera: Braconidae), were topically treated with diflubenzuron (Dimilin®), a chitin synthesis inhibitor, at 100, 500 and 1000 ppm. Each dosage was initiated during the parasite's egg stage, each of four larval stages and the pupal stage (pharate pupa + pharate adult). Adults emerging from all immature stages treated with the two higher dosages were significantly (P < 0.05) fewer than the controls. Developmental stages, in decreasing order of susceptibility to diflubenzuron were: egg, first instar, pharate adult, pharate pupa and larval stages two to four. Abnormally developed ovipositors occurred in some adults which emerged from eggs, pharate pupa and pharate adults that were treated with the two higher concentrations. Such females were unable to successfully locate and parasitize hosts. Females with normal ovipositors which emerged from treated eggs and pharate adults were able to find and attack hosts but had significantly fewer eggs in their ovaries and oviposited less than the controls. The F1 eggs laid by these females developed normally and survived to adulthood with no apparent abnormalities.