The mass concentration of uranium in water samples collected from the Bathinda districtof Punjab state, India, was estimated using the laser fluorimetric technique. The studyregion has shown a pronounced number of cancer cases in the recent period. The study aimsto calculate the human radiological risk and chemical toxicity associated with uraniumconsumption through drinking water. The mass concentration of uranium was found to varyfrom 0.48 to 571.7 μg/l with a mean value of 84.70 μg/l. The radiological risk due toconsumption of uranium through contaminated drinking water was observed to be in the rangeof 1.34 × 10-6 to 1.60 × 10-3 with a mean value of2.37 × 10-4. The chemical toxicity was found to vary from 0.04–43.11µg.kg-1.day-1 with a mean value of 6.38µg.kg-1.day-1.