A laser stripe sensor has two kinds of calibration methods. One is based on the homography model between the laser stripe plane and the image plane, which is called the one-step calibration method. The other is based on the simple triangular method, which is named as the two-step calibration method. However, the geometrical meaning of each element in the one-step calibration method is not clear as that in the two-step calibration method. A novel mathematical derivation is presented to reveal the geometrical meaning of each parameter in the one-step calibration method, and then the comparative study of the one-step calibration method and the two-step calibration method is completed and the intrinsic relationship is derived. What is more, a one-step calibration method is proposed with 7 independent parameters rather than 11 independent parameters. Experiments are conducted to verify the accuracy and robust of the proposed calibration method.