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laser-assisted polar body biopsy

Cambridge University Press (1)

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Chapter 14 - Preimplantation genetic diagnosis for sex-linked diseases and sex selection for non-medical reasons
from Section 2 - Procedures used in preimplantation genetic diagnosis
- By Caroline Mackie Ogilvie, Paul N. Scriven
Edited by Joyce Harper, University College London
Book:

Preimplantation Genetic Diagnosis

Published online:

09 November 2009

Print publication:

28 May 2009, pp 230-236
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Summary

Laser-assisted polar body biopsy is best accomplished when the oocyte is affixed to the holding capillary with the first polar body at 12 O'clock position and the second polar body located right of the first one but in the same focal plane. In contrast to embryonic blastomeres, polar bodies are rather small and do not require any special pretreatment like hypo-osmotic swelling or pronase digestion. The size and position of openings drilled in the zona pellucida (ZP) can influence further embryonic development and in particular the mode of hatching at the blastocyst stage. The presence of a cytoplasmic bridge between the first as well as the second polar body and the oocyte can impose during biopsy. A frequent problem in analysis of the first polar body is high degree of fragmentation observed in human first polar bodies.