Managing weeds in lawns using cultural practices such as mowing, irrigation, and fertilization may be important in integrated management. A field experiment evaluated the impact of cultural factors on weed populations in St. Augustinegrass turf. Irrigation treatments were daily to replace evapotranspiration (“Daily”), weekly to saturate the root zone only when wilted (“Conditional Weekly”), and as needed to saturate the root zone after severe wilt (“Severely Wilted”). Averaged over 3 yr, the Daily, Conditional Weekly, and Severely Wilted irrigation treatments resulted in 30, 6, and 2% dollarweed cover, respectively. Except for dollarweed and mat lippia, the largest cover of other weeds was under Severely Wilted irrigation. Fertilization rates were 0, 14, or 28 g N m−2 yr−1; in 2002, the highest fertilization rate had the smallest cover of weeds other than dollarweed. Mowing heights were 64, 89, or 114 mm; in 2003, the shortest mowing height had the smallest cover and dry weight of weeds other than dollarweed. After 3 yr of cultural management, most plots were excessively weedy, and turfgrass quality for all cultural management-treatment combinations, in the absence of herbicides, was unacceptable.