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membrane anchors

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1 results

Targeting and processing of membrane-anchored YFP fusion proteins to protein storage vacuoles in transgenic tobacco seeds
Ka Leung Fung, Yin Fun Yim, Yu Chung Tse, Yansong Miao, Samuel S.M. Sun, Liwen Jiang
Journal:

Seed Science Research / Volume 15 / Issue 4 / December 2005

Published online by Cambridge University Press:

22 February 2007, pp. 361-364
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Seeds that store proteins in protein storage vacuoles are attractive bioreactors for producing and storing large amounts of pharmaceutical proteins. However, foreign proteins expressed in transgenic plants are subjected to the delivery and modification processes present within plant cells. Here, it is demonstrated that unique membrane sequences deliver a yellow fluorescent protein (YFP) to the seed protein storage vacuoles in transgenic tobacco (Nicotiana tabacum L.) plants, where the YFP is then separated from its membrane anchors. This precise targeting and separation is required for the successful delivery of useful proteins to seed protein storage vacuoles for their stable accumulation in transgenic crops.