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methylation-sensitive restriction enzymes

European Journal of Phycology (1)

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DNA methylation during antheridial filament development and spermiogenesis in Chara vulgaris (Charophyceae) analysed by in situ nick-translation driven by methylation-sensitive restriction enzymes
MARIA J. OLSZEWSKA, DOROTA GERNAND, MIROSŁAW GODLEWSKI, AGNIESZKA KUNACHOWICZ
Journal:

European Journal of Phycology / Volume 32 / Issue 3 / August 1997

Published online by Cambridge University Press:

01 August 1997, pp. 287-291

Print publication:

August 1997
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DNA of Chara vulgaris thalli contains target sequences for the methylation-sensitive restriction enzymes, Sau3A, HpaII and MspI. These enzymes were used with the in situ nick-translation radioactive technique to evaluate by grain counting in autoradiograms changes in cytosine methylation during antheridial filament development and spermiogenesis. In successive generations of dividing antheridial filaments the level of DNA methylation gradually increases, then decreases slightly in the early stages of spermiogenesis. Spermatozoids display the highest level of DNA methylation.