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motor protein

Protein Science (1)

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Cloning and expression of kinesins from the thermophilic fungus Thermomyces lanuginosus
ROMAN SAKOWICZ, SAM FARLOW, LAWRENCE S.B. GOLDSTEIN
Journal:

Protein Science / Volume 8 / Issue 12 / December 1999

Published online by Cambridge University Press:

01 December 1999, pp. 2705-2710

Print publication:

December 1999
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The motor domain regions of three novel members of the kinesin superfamily TLKIF1, TLKIFC, and TLBIMC were identified in a thermophilic fungus Thermomyces lanuginosus. Based on sequence similarity, they were classified as members of the known kinesin families Unc104/KIF1, KAR3, and BIMC. TLKIF1 was subsequently expressed in Escherichia coli. The expression level was high, and the protein was mostly soluble, easy to purify, and enzymatically active. TLKIF1 is a monomeric kinesin motor, which in a gliding motility assay displays a robust plus-directed microtubule movement up to 2 μm/s. The discovery of TLKIF1 also demonstrates that a family of kinesin motors not previously found in fungi may in fact be used in this group of organisms.