A modified nutrient solution, used to study lettuce cultivar response to thiobencarb, provided an excellent medium for lettuce growth and is an alternative to other nutrient solutions. Leaf dry weight was inhibited 50% by 3- and 5-μM thiobencarb in ‘Dark Green Boston’ and ‘Great Lakes 366’, respectively, while ‘Bibb’, ‘Slobolt’ and ‘Valmaine’ responded intermediately. Growth inhibitions and herbicide injury symptoms, which included abnormal growth and stunted secondary leaves, were similar in both nutrient solution and soil assays. Due to its ease of preparation and elimination of the variability associated with soil assays, the nutrient solution may be considered a superior alternative to soil assays.