Fascioliasis is a zoonotic disease caused by liver flukes transmitted by freshwater lymnaeid snails. Donkey and horse reservoir roles have been highlighted in human endemic areas. Liver fluke infection in mules has received very limited research. Their role in disease transmission, epidemiological importance and Fasciola hepatica pathogenicity are studied for the first time. Prevalence was 39.5% in 81 mules from Aconcagua, and 24.4% in 127 from Uspallata, in high-altitude areas of Mendoza province, Argentina. A mean amount of 101,242 eggs/mule/day is estimated. Lymnaeids from Uspallata proved to belong to ribosomal DNA internal transcribed spacer (ITS) markers ITS-1 and ITS-2 combined haplotype 3C of Galba truncatula. These lymnaeids were experimentally susceptible to infection by egg miracidia from mules. Infectivity, number of cercariae/snail and shedding period fit the enhanced F. hepatica/G. truncatula transmission pattern at very high altitude. This indicates that the mule is able to maintain the F. hepatica cycle independently. Individual burdens of 20 and 97 flukes were found. Mule infection susceptibility is intermediate between donkey and horse, although closer to the latter. Anatomo-pathology and histopathology indicate that massive infection may cause mule death. Haematological value decreases of red blood cells, haemoglobin, leucocytes and lymphocytes indicate anaemia and strong immunosuppression. Strongly increased biochemical marker values indicate liver function alterations. The mule probably played a role in the past exchanges with Chile and Bolivia through Mendoza province. Evidence suggests that mules could contribute to the spread of both F. hepatica and G. truncatula to human fascioliasis-endemic areas in these countries.

In this article we detail the methodology developed to construct an efficient interface description technique — the robust conservative level set (RCLS) — to simulate multiphase flows on mixed-element unstructured meshes while conserving mass to machine accuracy. The approach is tailored specifically for industry as the three-dimensional unstructured approach allows for the treatment of very complex geometries. In addition, special care has been taken to optimise the trade-off between accuracy and computational cost while maintaining the robustness of the numerical method. This was achieved by solving the transport equations for the liquid volume fraction using a WENO scheme for polyhedral meshes and by adding a flux-limiter algorithm. The performance of the resulting method has been compared against established multiphase numerical methods and its ability to capture the physics of multiphase flows is demonstrated on a range of relevant test cases. Finally, the RCLS method has been applied to the simulation of the primary breakup of a flat liquid sheet of kerosene in co-flowing high-pressure gas. This quasi-DNS/LES computation was performed at relevant aero-engine conditions on a three-dimensional mixed-element unstructured mesh. The numerical results have been validated qualitatively against theoretical predictions and experimental data. In particular, the expected breakup regime was observed in the simulation results. Finally, the computation reproduced faithfully the breakup length predicted by a correlation based on experimental data. This constitutes a first step towards a quantitative validation.

This article verifies the existence of three different and complementary geographies in the 1790–1830 period, a stage in the Catalan industrialization process that has deserved little attention among historians. The first one, of manual spinning by means of an improved jenny –locally named bergadana– in inner Catalonia; a second one of improved water-frame spinning in Manresa and the surrounding villages; and, finally, a third one of wood mule-jenny in Barcelona and some towns of the seaside district of the Maresme. These three areas were characterized by using distinctive power sources and producing different types of thread; they also had different wage levels; but they were complementary until the 1830's when the introduction of new mechanical instruments required important and innovative organization changes. The article ends with two analitical case studies: Salient as a typical example of manual spinning, and Manresa as a case of water frame spinning.