Sublittoral and profundal chironomid community structure, depth distribution and relationship to the trophic level of the waters were investigated in Lake Vico (Central Italy) during 1985-1986. PCA analysis indicated a depth-dependent group of true profundal stations at 20, 30 and 40 m depths, characterized by Micropsectra, Procladius, Microtendipes, Tanytarsus and Paratendipes, and a group of 10 m stations, characterized by Cryptochironomus, Polypedilum bicrenatum gr., Cladopelma lateralis gr. and C. laccophila gr., representing the sublittoral features of this depth. In agreement with the chemical data, the chironomid community indicated a mesotrophic condition of Lake Vico, with a tendency towards an increasing trophic level in the deepest zone. A significant role in revealing future trophic changes in the lake may be attributed to the key-indicator Micropsectra.