In this paper, we present an experimental and numerical analysis of the influence of gear center distance variation and misalignment amplitude on dynamic response of single stage spur gear transmission systems. The experimental part is conducted on a specific test bench conceived and constructed at LASEM and composed essentially of a motor, a test gearbox and a receptor. It allows the measurement of the acceleration on the motor and receptor bearing shafts for different values of gear center distances and misalignment amplitudes. The numerical study is based on a 3D non-linear model with 36 degrees of freedom including torsional, flexural and axial displacements of the gear-shaft-bearing system. The equations of motion and the contact problem are solved simultaneously using an original procedure by coupling a normal contact algorithm and a numerical Hilbert-Hughes-Taylor schema. Agreement between measurements and calculations is satisfactory and illustrates the influence of these mounting errors on the dynamic behavior of Narrow-Faced Spur Gear.