This study examines levels of occupational stress, quality of life, work-related stressors, and coping strategies among senior police officers in Brazil. A quantitative questionnaire survey conducted among 418 senior members of the São Paulo Police Force reveals that high-ranking Brazilian police officers perceive their profession as being very stressful. A large proportion (43%) of police officers are found to have significant stress symptoms. A greater proportion of females (54%) than males (40%) are found to have stress. The most frequently reported stressor is interaction with other departments within the police force. Quality of life is found to be deficient in the ‘professional’ and ‘health’ areas. This study is the first to show a clear association between high levels of emotional stress and poor quality of life in Brazilian police officers. The large number of stress symptoms and poor quality of life identified in the present study indicates that there is a need for preventive actions inside the Brazilian police force to motivate lifestyle changes, improve stress-management skills, and promote a better quality of life among high-ranking police officers.