Political opposition has long been one of the most dramatically understudied elements of real-world politics in contemporary democratic and authoritarian regimes. The past decade or so has, however, witnessed an upsurge of new opposition research that begs for a major state-of-the-field review. Interestingly, recent scholarship has focused more on manifestations of opposition in authoritarian and hybrid than in democratic systems, which indicates a latent reconceptualization of political opposition (setting aside older distinctions between regime-loyal opposition and regime-challenging forms of resistance, dissidence and contestation). With a focus on party-based forms of opposition, which have been widely considered to mark the most effective form of opposition, this review article takes stock and highlights key issues for future research as well as some inherent obstacles to the emergence of a more integrated field of cross-regime opposition studies.