Inadequate human actions are still the main cause of all reported accidents and incidents on board ships. The aim of this study is to explore the influence of safety resource activities and other exploratory variables on seafarers’ safety behaviour. For this study, a series of open-ended interviews were conducted to gain insight into how shipping organisations struggle with this issue. Following the interviews, a questionnaire was prepared and distributed to professional seafarers. Two exploratory factor analyses were conducted to identify the underlying factor structure and five factors emerged. Furthermore, three hierarchical multiple regression analyses were conducted to examine the influence of the factors obtained, namely, Company safety orientation and Additional safety incentives on seafarers’ perceived Safety awareness, Job satisfaction, Risk acceptance and other exploratory variables. The study shows that the perceived resource-related activities of the organisation and the demographic characteristics of the respondents can influence the safety behaviour of seafarers.