According to our previous results, the conjugacy class of the involution induced by the complex conjugation in the homology of a real non-singular cubic fourfold determines the fourfold up to projective equivalence and deformation. Here, we show how to eliminate the projective equivalence and obtain a pure deformation classification, that is, how to respond to the chirality problem: which cubics are not deformation equivalent to their image under a mirror reflection. We provide an arithmetical criterion of chirality, in terms of the eigen-sublattices of the complex conjugation involution in homology, and show how this criterion can be effectively applied taking as examples M-cubics (that is, those for which the real locus has the richest topology) and (M−1)-cubics (the next case with respect to complexity of the real locus). It happens that there is one chiral class of M-cubics and three chiral classes of (M−1)-cubics, in contrast to two achiral classes of M-cubics and three achiral classes of (M−1)-cubics.