Müller cells are highly permeable to potassium
ions and play a major role in maintaining potassium homeostasis
in the vertebrate retina during light-evoked neuronal activity.
Potassium fluxes across the Müller cell's membrane
are believed to underlie the light-evoked responses of
these cells. We studied the potassium currents of turtle
Müller cells in the retinal slice and in dissociated
cell preparations and their role in the genesis of the
light-evoked responses of these cells. In either preparation,
the I–V curve, measured
under voltage-clamp conditions, consisted of inward and
outward currents. A mixture of cesium ions, TEA, and 4-AP
blocked the inward current but had no effect on the outward
current. Extracellular cesium ions alone blocked the inward
current but exerted no effect on the photoresponses. Extracellular
barium ions blocked both inward and outward currents, induced
substantial depolarization, and augmented the light-evoked
responses, especially the OFF component. Exposing isolated
Müller cells to a high potassium concentration did
not cause any current or voltage responses when barium
ions were present. In contrast, application of glutamate
in the presence of barium ions induced a small inward current
that was associated with a substantially augmented depolarizing
wave relative to that observed under control conditions.
This observation suggests a role for an electrogenic glutamate
transporter in generating the OFF component of the turtle
Müller cell photoresponse.