Early-life nutrition affects calf development and thus subsequent performance. The aim of this study was to examine the effect plane of nutrition on growth, feeding behaviour and systemic metabolite concentrations of artificially reared dairy bull calves. Holstein-Friesian (F; n=42) and Jersey (J; n=25) bull calves with a mean±SD age (14±4.7 v. 27±7.2 days) and BW (47±5.5 v. 33±4.7 kg) were offered a high, medium or low plane of nutrition for 8 weeks using an electronic feeding system which recorded a range of feed-related events. Calves were weighed weekly and plasma samples were collected via jugular venipuncture on weeks 1, 4 and 7 relative to the start of the trial period. The calves offered a high plane of nutrition had the greatest growth rate. However, the increased consumption of milk replacer led to a reduction in feed efficiency. Holstein-Friesian calves offered a low plane of nutrition had the greatest number of daily unrewarded visits to the feeder (P<0.001). β-hydroxybutyrate (BHB) concentrations were greater in F calves on a low plane of nutrition (P<0.001). Although there was no effect of plane of nutrition, BHB concentrations in F calves increased before weaning, concomitant with an increase in concentrate consumption. Urea concentrations were unaffected by plane of nutrition within either breed. Jersey calves on a low plane of nutrition tended to have lower triglycerides than those on a high plane (P=0.08), but greater than those on a medium plane (P=0.08). Holstein-Friesian calves offered a high plane of nutrition tended to have greater triglyceride concentrations than those on a medium plane (P=0.08). Triglycerides increased from the start to the end of the feeding period (P<0.05), across both breeds. A medium plane of nutrition resulted in a growth, feeding behaviour and metabolic response comparable with a high plane of nutrition in pre-weaned bull calves of both F and J breeds.

Haematological profiles indicate the health status of an animal and can be used to identify sub-clinical stress responses. The objectives of the study were to examine (i) the effect of breed and plane of nutrition, on haematological profiles of artificially reared Holstein-Friesian and Jersey bull calves in response to gradual weaning, and (ii) the effect of breed on immune response genes in bovine whole blood using real-time quantitative PCR. Holstein-Friesian and Jersey bull calves were group housed indoors and individually fed using an automatic feeder. They were allocated to a high, medium or low plane of nutrition, based on milk replacer (MR) and concentrate. The nutrition treatments were calculated using National Research Council guidelines in order to achieve a high, medium or low growth rate for each respective breed. During the weaning phase MR was gradually reduced over a 14-day (d) period (d −13 to d 0). Calves were blood sampled on d −14, −6, −3, 0, 1, 3, 8 and 14 relative to weaning (d 0) for subsequent haematological analysis. On d −14, 1 and 8, a subset of eight Holstein-Friesian calves randomly selected from the medium nutrition treatment and eight Jersey calves randomly selected from the high nutrition treatment, were blood sampled for gene expression profiling, targeting biomarkers of weaning stress. These two treatment groups were chosen to examine the effect of breed on expression of the genes of interest, as energy intake and animal performance were similar. There was no effect of breed×plane of nutrition interaction nor effect of plane of nutrition on any variable measured (P>0.05). Gradual weaning produced differential biological responses in the two breeds evidenced by breed×time interactions for lymphocyte, monocyte and red blood cell number, plasma haemoglobin and haptoglobin concentrations (P<0.05). The typical stress response consisting of neutrophilia and lymphopaenia was not observed for any treatment. An immune response to gradual weaning was observed as the relative gene expression level of the pro-apoptotic gene, Fas, increased on d 1 relative to d −14 (P<0.05). Relative gene expression levels were greater in Jersey calves compared with Holstein-Friesian for the pro-inflammatory cytokine CXCL8 (P=0.05) and the glucocorticoid receptor, GRα (P<0.05). The increased levels of these transcripts suggest that Jersey calves may have a more sensitive immune system compared with Holstein-Friesian.

The aim of this study was to determine the effects of restricted feeding before puberty on reproduction, lactation and offspring growth performance in replacement ewe lambs over two breeding seasons. At weaning, 41 Dorset ewe lambs were assigned to one of three diets: an ad libitum control diet with medium-quality forage (MQF; 13.3% crude protein (CP), 1.81 Mcal metabolizable energy per kg, 42.8% ADF; diet A-MQF); a restricted diet with the same forage as A but less feed concentrate (diet R-MQF); or a high-quality forage (HQF) diet (14.8% CP, 2.15 Mcal ME/kg, 34.7% ADF; diet F-HQF). The quantity of concentrate offered to the group R-MQF and F-HQF ewe lambs was adjusted to obtain 70% of the control ewe lambs’ growth rate. The diets were offered for 75 days following weaning to cover the allometric phase of mammary gland development. Prepubertal restriction did not affect (P > 0.10) the gestation rate, number of lambs born or the body weight and body condition score of ewes at lambing or at the end of lactation. Ewes from groups R-MQF and F-HQF tended to produce more milk during their first lactation compared to those from group A-MQF (P = 0.07). During the second lactation, groups R-MQF and F-HQF had better standardized milk production than group A-MQF (P < 0.05), and group R-MQF produced more milk than group F-HQF (P < 0.05). Milk fat and protein content were not affected by treatments (P > 0.10) Fat and protein yield were affected by treatments only at the second lactation (P < 0.10 and P < 0.05, respectively). Lamb birth and weaning weights were not affected by prepubertal restriction of feeding in their mother (P > 0.10). However, the average daily gain of second breeding season lambs was higher for the R-MQF group than the F-HQF group (P < 0.05), and a similar trend was observed for total gain (P < 0.10). Restricted feeding before puberty does not impair future reproductive performance; however, it has a positive impact on lactation and on lambs’ growth performance.

The aim of this study was to determine the effects of restricted feeding before puberty on growth performance and mammary gland development in replacement ewe lambs. At weaning, 72 Dorset ewe lambs were assigned to one of the three diets: an ad libitum control diet with medium-quality forage (MQF; diet A-MQF); a restricted diet with the same forage as A, but less feed concentrate (diet R-MQF); or a high-quality forage (HQF) diet (diet F-HQF). The quantity of concentrate offered to the group R-MQF and F-HQF ewe lambs was adjusted to obtain 70% of the control ewe lambs’ growth rate. The diets were offered for 75 days after weaning to cover the allometric phase of mammary gland development. During this period, average daily gain (ADG) was 223 and 229 g/day for groups R-MQF and F-HQF, respectively, compared to 305 g/day for group A-MQF (P < 0.0001). At the end of this period, 28 ewe lambs were slaughtered and their mammary gland was collected. Parenchymal fresh tissue weight tended to be higher for groups R-MQF and F-HQF compared to group A-MQF (P = 0.09). Stroma weight was greater (P < 0.05) for the group A-MQF ewe lambs than for those in the other treatments. Total DNA and total protein in parenchymal tissue tended to be greater for groups R-MQF and F-HQF (P = 0.09 and P = 0.07, respectively). Dry fat-free tissue was greater for groups R-MQF and F-HQF (P < 0.05). The remaining ewe lambs were fed the same haylage and barley diet until their first lambing. During this period, compensatory growth was observed. ADG was greater (P < 0.01) for groups R-MQF and F-HQF than for group A-MQF, and feed conversion was improved (P < 0.01) for groups R-MQF and F-HQF compared with the control, whereas the dry matter intake was similar for all groups. Live body weight, loin eye depth and backfat depth at breeding and around lambing were similar for all groups. The results of this study suggest that restricted feeding before puberty improves mammary gland development without compromising growth performance in ewe lambs.

The effect of plane of nutrition on the timing of the moult in cashmere goats was investigated. Three groups of mature does were individually offered food to supply 0·8 (L, no. = 14), 1·2 (M, no. = 14) or 20 (H, no. = 13) times their estimated maintenance energy requirements from mid December until mid May. Two other groups of goats which were shorn in mid January, were offered 1·2 times maintenance requirements from mid December until mid March when food levels were increased to either 1·6 M (SL, no. = 6) or ad libitum (SAL, no. = 6). In mid May mean live weights for treatments L, M, H, SL and SAL were 36·0, 41·5, 46·9, 33·0 and 43·1 kg (average s.e.d. = 2·27, P < 0·001).

The pattern of moult was described by changes in a subjectively assessed moult score, the proportion of follicles with brushes and staple length. The loss of brushes, indicating the start of the moult, began from the primary follicles on days 143, 131, 76, 150 and 129 (average s.e.d. = 13·5, P < 0001), and from the secondary follicles on days 141, 128, 104, 144 and 125 (average s.e.d. = 14·1, P = 0·053) for treatments L, M, H, SL and SAL respectively. The loss of brushes from primary and secondary follicles was completed on average, by days 194 and 206 of the experiment respectively, and there were no differences between treatments. Moult score and staple length data confirmed the conclusion that lowering the plane of nutrition delayed the start of the moult. No additional effect of fleece removal in the shorn treatments was observed.

Changes in circulating levels of plasma prolactin, thyroxine, tri-iodothyronine, insulin, insulin-like growth factor-1 and growth hormone were related to seasonal changes in daylength and plane of nutrition. Their rôle in the control of the moult is discussed.

Manipulation of the nutrition of cashmere goats in early spring has the potential to delay and increase the synchrony of fibre moult and to improve the efficiency of cashmere harvesting.

Seventy high genetic merit Holstein heifers were used in two experiments to investigate (1) the effects of plane of nutrition and diet type during the pre-pubertal period and (2) the effects of plane of nutrition during the post-pubertal period on metabolic hormone concentrations, growth and milk production. In experiment 1, treatment 1 and 2 heifers were given food to achieve a live-weight gain of 0·70 and 0·95 kg/day from 3 to 10 months of age on a grass silage based diet, while treatment 3 heifers were given food to achieve 0·95 kg/day on a barley straw/concentrate diet. During the pre-pubertal period, heifers reared on treatment 1 had significantly higher growth hormone(GH) concentrations (ng/ml per 1 h) than heifers reared on treatment 2 (P < 0·01) and had significantly lower insulin concentrations than heifers reared on treatment 3 (P < 0·01). Heifers reared on treatment 1 had significantly lower insulin-like growth factor 1 (IGF-1) concentrations than those reared on treatment 3 (P < 0·01). At 10 months of age heifers reared on treatment 1 were of lower condition score (P < 0·01) than those on treatment 2 and had a smaller heart girth diameter (P < 0·01) than those on treatments 2 and 3. During the first lactation, milk yield and composition produced by the heifers was not significantly affected by treatment. In experiment 2, treatment A heifers were given, from 14 to 24 months of age, a low plane of nutrition to allow a live-weight gain of 0·65 kg/day on a grass silage and grass based diet during the winter and summer periods respectively. Treatment B heifers were kept on a high plane of nutrition to allow a live-weight gain of 0·90 kg/day on the same forage along with concentrate supplementation. During the rearing period, GH and IGF-1 concentrations were not significantly affected by treatment. Treatment A heifers weighed less before calving (P < 0·05), had a lower condition score (P < 0·01), and had a smaller heart girth diameter (P < 0·01) than those on treatment B. During the first 10 weeks of lactation, heifers on treatment A had a higher silage dry matter intake and lost less weight (P < 0·05) than those on treatment B, however, by 20 weeks of lactation these effects had disappeared. Milk yield and composition during the first lactation were not significantly affected by treatment. Overall, the findings of experiments 1 and 2 did not show any beneficial effects of higher weights at first calving in high genetic merit Holsteins and therefore indicate that accelerated growth in the pre- or post-pubertal period may not be required.

The influence of plane of nutrition and diet on the eating quality of fresh pork was investigated in a study involving 721 animals. Boars and gilts of three genotypes (0, 0·25 and 0·50 Duroc inclusion level) were reared from 30 to 90 kg on seven feeding regimens (combinations of diet formulation and feeding level) to achieve different rates of lean and fat tissue growth during two growth periods (30 to 60 or 75 kg; 60 or 75 kg to 90 kg), respectively. A diet of conventional energy and protein (CEP, 14·2 MJ/kg digestible energy, 205 g/kg crude protein, 10 g/kg lysine) was given using combinations of ad libitum and restricted feeding to produce six treatment groups with variation in lean and fat growth rates. An additional treatment group was given food ad libitum on a higher energy and lower protein diet (HELP, 14·7 MJ/kg digestible energy, 166 g/kg crude protein, 7·0 g/kg lysine) between 30 and 90 kg. Dissected carcass composition at 90 kg was predicted from equations based on P2 fat depth, which were developed from full-side and ham joint dissections on sub-samples of animals. Representative sub-samples of animals were dissected at start (30 kg) and at interim weights (60 or 75 kg) to allow lean and subcutaneous fat growth rates to be calculated for all or parts of the growth period. The feeding regimes produced substantial variation in live-weight gain (DLWG) (744 to 914 g/day) and lean tissue growth rate (LTGR 345 to 417 g/day) and subcutaneous fat growth rate (SFGR 81 to 97 g/day), between 30 and 90 kg, and in longissimus dorsi intramuscular fat content (10·37 to 23·87 g/kg). Pigs given the HELP diet had the highest intramuscular fat and the best eating quality. Pigs offered the CEP diet ad libitum throughout the growth period produced more tender but less juicy meat than those given food restrictedly (0·8 or 0·9 of ad libitum). The correlations between DLWG, LTGR and SFGR for the whole or parts of the growth period and sensory characteristics, although often positive, were generally low, suggesting weak relationships.

Bulls (½ Blonde d'Aquitaine ⅜ Charolais) were used in a 2 × 3 factorial design experiment with two planes of nutrition in the finishing period and three slaughter weights. High and low planes of nutrition were based on diets with similar forage to concentrate ratio (0·40 of dry matter (DM)) offered ad libitum or at 0·78 of ad libitum DM intake at equal live weight respectively. The target slaughter live weights were 550, 625 and 700 kg. Twelve bulls were taken to each slaughter point and an additional five animals were killed as a pre-experimental slaughter group. Mean initial live weight was 412 (s.e. 5·3) kg at a mean age of 342 (s.e. 2·5) days. No significant interactions were found between the main factors. For the high and low planes, live-weight and estimated carcass gains were 1251 and 989 (s.e. 47·7), and 816 and 668 (s.e. 35·3) g/day respectively, the reduction in gains being similar to the proportional degree of nutritional restriction. Plane of nutrition had no effect on live-weight or carcass gain per unit of energy intake. The low plane of nutrition produced significant decreases in body cavity fat depots, subcutaneous fat in the sample joint and increased the proportions of both saleable beef and high-priced joints in the carcass.

With increase in slaughter weight, energy intake per unit of live weight0·75 and rates of both live-weight and carcass gain tended to decline. The dressing proportions were 583,579 and 609 (s.e. 9·1) g/kg for the slaughter live weights of 550, 625 and 700 kg. Carcass conformation improved while fat depots in the body cavity and estimated concentration of separable fat in the carcass increased with increase in slaughter weight, and both estimated lean and bone concentrations decreased. Forequarter as a proportion of total side tended to increase with increase in slaughter weight. Slaughter weight had no effect on concentration of saleable meat or ultimate pH of carcass muscle. It is concluded that bulls of this genotype can be taken to high slaughter weights on diets having a relatively high proportion of forage as grass silage, with high rates of growth and acceptable carcass leanness.

A total of 252 lambs was subjected to two contrasting growth rates (high and low) for 7 weeks commencing at 6 weeks of age, factorially arranged with two contrasting growth rates (high and low) from 13 weeks to slaughter. Growth rate was controlled either by weaning lambs at 6 weeks and feeding indoors throughout on differing levels of a concentrate diet (84 lambs in experiment 1) or by grazing at differing sward heights up to weaning at 13 weeks after which weaned lambs were grazed at differing sward heights and where necessary housed from early October and offered differing levels of concentrate and silage (168 lambs in experiment 2). Lamb slaughter weights on each growth rate treatment covered the range 40 to 48 kg live weight.

Increasing the growth rate between 6 and 13 weeks from 213 to 320 glday in experiment 1 and from 191 to 291 g/day in experiment 2 did not significantly affect the fat depth measured at various locations throughout the carcass, the weight of perinephric and retroperitoneal fat, fat grade or the total lipid content of soft tissue dissected from the shoulder joint when lambs were taken to slaughter weights. Increasing the growth rate between 13 weeks and slaughter from 136 to 338 g/day in experiment 1 and from 99 to 146 g/day in experiment 2 significantly increased the depth of carcass fat and fat grade but, though lipid content of the tissue in the shoulder also increased, this difference was not significant.

Carcass fat depth, perinephric and retroperitoneal fat, fat grade and lipid content of the shoulder tissue increased as slaughter weight increased. The effect of carcass weight on carcass fat was greater on the high plane of feeding from 13 weeks than on the low plane. For example, mean fat depth increased by 0·52 mm/kg increase in carcass weight on the high plane compared with 0·13 mm/kg on the low plane.

Comparing the effect of plane of feeding and carcass weight on carcass fat depth the high finishing plane of feeding had an effect equivalent to an increase of 3·8 kg carcass weight. The equivalent figure for the depth of tissue over the 12th rib was 2·8 kg while in relation to fat grade the effect was smaller at 1·0 kg carcass weight.

During a series of routine experiments to evaluate 16 foods offered to wether sheep, conventional measurements of digestibility were augmented by measurement of fractional outflow rates (FOR) of solids (ks) and liquid (k1) from the rumen and urinary allantoin nitrogen excretion (UAN) as an index of microbial yield. Fixed (1 kg) amounts of ‘dry’ foods were offered to sheep ranging in weight from 42 to 89 kg which permitted a retrospective analysis of plane of nutrition effects on diet digestibility, ks, k1 and UAN. Each diet was simultaneously offered to three sheep (one each of large, medium and small size). Smaller sheep had significantly higher FOR, significantly lower apparent digestibilities of dry matter and significantly higher UAN (g/kg digestible organic matter (DOM)). Higher UAN (g/kg DOM) indicated higher microbial energetic efficiencies with the smaller sheep.