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programmed ribosomal frame-shifting

RNA (1)

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Development of a tRNA-dependent in vitro translation system
RICHARD J. JACKSON, SAWSAN NAPTHINE, IAN BRIERLEY
Journal:

RNA / Volume 7 / Issue 5 / May 2001

Published online by Cambridge University Press:

04 May 2001, pp. 765-773

Print publication:

May 2001
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A method is described for depleting rabbit reticulocyte lysates and wheat germ extracts of endogenous tRNAs by affinity chromatography using a matrix generated by coupling ethanolamine to epoxy-activated Sepharose 6B. Greater than 90% depletion of tRNA is achieved with the result that translation becomes in effect absolutely dependent on added tRNA. This depletion procedure should prove very useful for studying the influence of tRNA concentration, and the spectrum of the tRNA population, on recoding events such as programmed frameshifting and readthrough of termination codons.