Oxytocin release, milking characteristics, and teat condition were investigated with reduced claw vacuum and pulsation settings compared to milking at regular settings with or without pre-stimulation. The reduced vacuum and pulsation settings during low milk flow are expected to protect the teat tissue before the occurrence of milk ejection at the start of milking, and at the end of milking during a potential overmilking period, i.e. at a milk flow <200 g/min. Seven cows were machine-milked either after a 60 s manual pre-stimulation, or without pre-stimulation and reduced vacuum and pulsation settings, or at full vacuum and normal pulsation during the start of milking. Plasma oxytocin (OT) concentration increased similarly in response to manual pre-stimulation and to both milking with reduced, or with full vacuum and pulsation settings, however delayed by 1 min if the cluster was attached without pre-stimulation. In all treatments OT concentrations remained elevated throughout milking. Milk flow curves were mostly non-bimodal at milkings after manual pre-stimulation and bimodal at milkings without pre-stimulation. The main milking time was shorter and average milk flow was higher during milking after pre-stimulation, but did not differ between treatments without pre-stimulation. Milk yields and peak flow rates were not affected by treatments. Either reduced or full vacuum settings were again applied during an intended overmilking from 200 to 100 g/min of milk flow towards the end of milk harvest. Pre-milking teat ultrasound cross sections were recorded one day before the experiment started. Post-milking ultrasound cross sections were performed at 15 min after each experimental milking. Teat wall thickness was increased after milking as compared to pre-milking but did not differ among treatments. In conclusion, OT release and milking performance are similar if milking is performed with pre-stimulation, or without pre-stimulation but reduced claw vacuum and b-phase during low milk flow.

Massive stars pulsate in various modes; radial and nonradial p-modes, g-modes, and strange modes including oscillatory convective (non-adiabatic g−) modes. Those modes are responsible for the light and velocity variations of β Cephei stars, slowly pulsating B (SPB) stars, and α Cyg variables. The instability mechanisms for these pulsations are discussed. We also discuss the relation between the evolution of massive stars and the excitation of strange modes, which are considered responsible for the pulsation in most of the α Cyg variables. The surface He and CNO abundances of hotter α Cyg variables seem to indicate that the Ledoux criterion of convection is better than the Schwarzschild criterion, although the latter is extensively used in stellar evolution computations.

Fundamental mode classical Cepheids have light curves which repeat accurately enough that we can watch them evolve (change period). The new level of accuracy and quantity of data with the Kepler and MOST satellites probes this further. An intriguing result was found in the long time-series of Kepler data for V1154 Cyg the one classical Cepheid (fundamental mode, P = 4.9 d) in the field, which has short term changes in period (≃20 minutes), correlated for ≃10 cycles (period jitter). To follow this up, we obtained a month long series of observations of the fundamental mode Cepheid RT Aur and the first overtone pulsator SZ Tau. RT Aur shows the traditional strict repetition of the light curve, with the Fourier amplitude ratio R1/R2 remaining nearly constant. The light curve of SZ Tau, on the other hand, fluctuates in amplitude ratio at the level of approximately 50%. Furthermore prewhitening the RT Aur data with 10 frequencies reduces the Fourier spectrum to noise. For SZ Tau, considerable power is left after this prewhitening in a complicated variety of frequencies.

The Blazhko effect is the conspicuous amplitude and phase modulation of the pulsation of RR Lyrae stars that was discovered in the early 20th century. The field of study of this mysterious modulation has recently been invigorated thanks to the space photometric missions providing long, uninterrupted, ultra-precise time-series data. In this paper I give a brief overview of the new observational findings related to the Blazhko effect, such as extreme modulations, irregular modulation cycles and additional periodicities. I argue that these findings together with dedicated ground-based efforts provide us now with a fairly complete picture and a good starting point to theoretical investigations. Indeed, new, unpredicted dynamical phenomena have been discovered in Blazhko RR Lyrae stars, such as period doubling, high-order resonances, three-mode pulsation and low-dimensional chaos. These led to the proposal of a new explanation to this century-old enigma, namely a high-order resonance between radial modes. Along these lines I present the latest efforts and advances from the theoretical point of view. Lastly, amplitude variations in Cepheids are discussed.