Lolium multiflorum, one of the most important temperate forage grasses in the world, is used in integrated crop-livestock systems and as a cover crop. However, it is also one of the main weeds in winter crops. The continuous use of glyphosate to manage this species has led to the selection of resistant biotypes (LOLMU-R), making it important to prevent the dispersal of these seeds. This study aimed to assess the recovery and germination of LOLMU-R that have passed through the digestive system of cattle. The experiments were carried out in metabolism cages, using a completely randomized design with six replications. The animals were given 12 112 seeds each, which were recovered from their faeces over a period of 6 days. Germination of the recovered seeds was assessed in a germination chamber and compared against a control (no animal passage). After germination, a glyphosate dose-response curve was constructed. The results obtained showed a total recovery of 1109 seeds (9.1%), with maximum recovery 2 days after ingestion, decreasing to almost zero on day 6. Germination declined linearly as a function of recovery time; however, 4 days after ingestion, germination potential was 18%. The dose-response curve proved the resistance of the recovered seeds. Cattle is a dispersal agent for LOLMU-R seeds, with animals requiring 7 days of quarantine before moving from one infested area to another.