Polyacrylamide gel disc electrophoresis of the salivary secretion from the tsetse, Glossina morsitans morsitans Westwood, showed 11 protein bands, four of which were characterized as glycoproteins. Sixteen amino acids and four sugars in the hydrolysates of saliva were identified. Thin layer chromatography showed five lipid classes and three phospholipids. The secretion is a clear, transparent, viscous fluid with a pH between 7.00 and 7.5. These findings are discussed in relation to the nutritional requirements of the metacyclic forms of trypanosomes that may be found in the salivary glands.
