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scrambled proteins

Protein Science (1)

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Analysis of the extent of unfolding of denatured insulin-like growth factor
JUI-YOA CHANG, WALTER MÄRKI, POR-HSIUNG LAI
Journal:

Protein Science / Volume 8 / Issue 7 / July 1999

Published online by Cambridge University Press:

01 July 1999, pp. 1463-1468

Print publication:

July 1999
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Insulin-like growth factor (IGF-1) contains three disulfide bonds. In the presence of denaturant and thiol catalyst, IGF-1 shuffles its native disulfide bonds and denatures to form a mixture of scrambled isomers. The composition of scrambled IGF varies under different denaturing conditions. Among the 14 possible scrambled IGF isomers, the yield of the beads-form isomer is shown to be directly proportional to the strength of the denaturing condition. This paper demonstrates a new approach to quantify the extent of unfolding of the denatured protein.