The double pot technique was used to study the effects of uptake site of fluridone {1-methyl-3-phenyl-5-[3-(trifluoromethyl—phenyl]-4(1H)-pyridinone} on early shoot and root development of soybean [Glycine max (L.) Merr. ‘Williams’] and cotton (Gossypium hirsutum L. ‘Acala SJ-1′). Soybean is susceptible and cotton is resistant to fluridone. Root uptake in soybean resulted in increased shoot length in the epicotyl and shoot apex, reduced shoot fresh weight, chlorosis of primary and trifoliolate leaves, as well as reduced root length, branching, and fresh weight. Shoot uptake resulted in reduced shoot length above the hypocotyl, reduced shoot weight, severe chlorosis of primary leaves, and inhibition of trifoliolate leaf formation. The reduction in the root length, branching, and weight was less by shoot uptake than by root uptake. In cotton, root uptake resulted in reduced shoot length in the epicotyl and shoot apex, slight reduction in shoot weight, no effect on chlorosis of cotyledon leaves, moderate to slight chlorosis of the true leaves, and no effect on root length, branching, or fresh weight. Shoot uptake resulted in reduced length of all shoot components and shoot weight, chlorosis of cotyledon leaves and the true leaves, and reduced root length, branching, and weight. Compared to soybean, cotton required much higher fluridone concentrations to show the above effects. It was generally concluded that in soybean, uptake by both the root and the shoot contributed significantly to the injury, whereas in cotton, shoot uptake was relatively more important.