The distribution pattern of s-triazine-resistant biotypes of common lambsquarters (Chenopodium album L. #3 CHEAL) and smooth pigweed (Amaranthus hybridus L. # AMACH) in Virginia was determined. Seeds were collected from suspected triazine-resistant biotypes of both species. Triazine resistance was confirmed by measuring chlorophyll fluorescence in the presence of atrazine [6-chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine]. Greenhouse bioassay with whole-plant material and a sinking leaf disc assay were also performed as further confirmation of triazine resistance. Triazine-resistant smooth pigweed was confirmed in 19 counties and common lambsquarters in eight counties in Virginia. Triazine-resistant smooth pigweed and common lambsquarters were located mostly in the northern and southwestern highlands of the state where there has been a long history of triazine use in no-till corn (Zea mays L.) production. S-triazine-resistant biotypes were also cross-resistant to other representative s-triazine and as-triazine herbicides but susceptible to the substituted urea herbicide diuron [N′-(3,4-dichlorophenyl)-N,N-dimethylurea].