This paper sought to determine whether common haematology and blood biochemistry values might assist in determining the relative welfare outcomes arising from the capture of red foxes (Vulpes vulpes) by treadle-snares, Victor Soft Catch® (VSC) #3 traps, cage traps, netting and sampling by shooting. Compared to all other capture methods and shooting, treadle-snared foxes had significantly higher mean albumin (ALB), creatine kinase (CK), red cell count (RCC), neutrophil to lymphocyte (N:L) ratio, sodium (Na), total protein (TP), white cell counts (WCC) and lower glucose (Gl). Treadle-snares were also associated with higher chloride (Cl), haemoglobin (Hb) and packed cell volume (PCV) than cage trapping and netting. Treadle-snares produced indicators of possible muscle damage, exertion and dehydration compared to cage and VSC traps. Cage trapping and netting produced lower indications of exertion, possible muscle damage and dehydration compared to both treadle-snares and VSC traps. These data do not support previous conclusions that due to similar injury scores, treadle-snares and VSC traps produced equivalent welfare outcomes. In restraining traps, injury and death sustained during capture are end-points of poor trapping welfare. Monitoring stress using physiological indicators allows the comparison of the relative potential for different capture techniques to cause pathological and pre-pathological states. As the response of physiological indicators to stress is not independent of time, accurate data on the duration of captivity and the relative intensity of struggling behaviour should be routinely collected when assessing the comparative humaneness of different trap devices.

Many studies of wildlife poaching acknowledge the challenges of detecting poaching activities, but few address the issue. Data on poaching may be an inaccurate reflection of the true spatial distribution of events because of low detection rates. The deployment of conservation and law enforcement resources based on biased data could be ineffective or lead to unintended outcomes. Here, we present a rigorous method for estimating the probabilities of detecting poaching and for evaluating different patrol strategies. We illustrate the method with a case study in which imitation snares were set in a private nature reserve in South Africa. By using an experimental design with a known spatial distribution of imitation snares, we estimated the detection probability of the current patrol strategy used in the reserve and compared it to three alternative patrol strategies: spatially focused patrols, patrols with independent observers, and systematic search patterns. Although detection probabilities were generally low, the highest proportion of imitation snares was detected with systematic search strategies. Our study provides baseline data on the probability of detecting snares used for poaching, and presents a method that can be modified for use in other regions and for other types of wildlife poaching.

Hunting is a primary driver of biodiversity loss across South-east Asia. Within Cambodia, the use of wire snares to capture wildlife is a severe threat in protected areas but there have been few studies of the behaviour of hunters from local communities. Here, we combine the unmatched count technique with direct questioning to estimate the prevalence of hunting behaviours and wildlife consumption amongst 705 households living within Keo Seima Wildlife Sanctuary, Cambodia. We assessed respondents’ knowledge of rules, and their perceptions of patrols responsible for enforcing rules. Estimates of hunting behaviour were variable: results from the unmatched count technique were inconclusive, and direct questioning revealed 9% of households hunted, and 20% set snares around farms to prevent wildlife eating crops. Hunting with domestic dogs was the method most commonly used to catch wildlife (87% of households owned dogs). Wild meat was consumed by 84% of households, and was most frequently bought or caught, but also gifted. We detected a high awareness of conservation rules, but low awareness of punishments and penalties, with wildlife depletion, rather than the risk of being caught by patrols, causing the greatest reduction in hunting. Our findings demonstrate the challenges associated with reliably estimating rule-breaking behaviour and highlight the need to incorporate careful triangulation into study design.

Hunting of wildlife is one of the major threats to biodiversity. For effective conservation programmes in countries where hunting and shifting agriculture are the main sources of subsistence, forest management should aim to reduce hunting pressure and forest exploitation. The presence of researchers has been promoted as one of the main ways to mitigate anthropogenic pressures on wildlife populations. Our aim was to test whether local management and the establishment of a research station had a role in decreasing forest exploitation by local people living adjacent to a recently protected area in south-east Madagascar. We interviewed local people from nine villages at various distances from the recently established research station of Ampasy, in the northernmost portion of the Tsitongambarika Protected Area, to explore how people use the forest, with a particular focus on hunting. We also performed transect surveys to estimate snare and lemur encounter rates before local forest management began, at the establishment of the research station, and 1 year after. The impact of local communities on the forest seems to have decreased since the beginning of forest management, with a further decrease since the establishment of the research station. Participants from villages not involved in the local management were more reluctant to declare their illegal activities. We conclude that a combination of local management and related activities (e.g. installation of a research station) can assist in temporarily reducing forest exploitation by local communities; however, community needs and conservation plans should be integrated to maintain long-term benefits.

Data on prices and quantities of wildlife on sale in markets are increasingly being used as indicators of the sustainability of bushmeat hunting, being relatively easy to collect. However, it is not clear how much can actually be inferred from trends in such data. This study analyses changes in price, quantity, method of capture and kill location of bushmeat species entering the Atwemonom bushmeat market in the city of Kumasi (Ghana) over the period 1987–2002, using data collected by an official of the Ghana Wildlife Department. The analysis was confined to the seven most commonly traded species and to the open season only, in order to maximize the reliability of the data. Over the period, there was an increase in real bushmeat prices, in the proportion of animals killed by means other than guns and the trade made up by grasscutters (Thryonomys swinderianus). A higher proportion of animals originated from distant areas, rather than from villages close to Kumasi. These trends are compatible both with depletion of the bushmeat resource and with an economically rational response by hunters to increasing prices. The analysis highlights both the utility and the limitations of detailed long-term market data as a tool for assessing the sustainability of wildlife hunting. Data on the origin of animals sold in the market and the gear type are necessary but not sufficient for understanding the drivers of changes in price and quantity of species on sale in markets; without complementary detailed local data on hunter behaviour, market data are of little value.

In protein transport between organelles, interactions of v- and t-SNARE proteins are required for fusion of protein-containing vesicles with appropriate target compartments. Mammalian SNARE proteins have been observed to interact with NSF and SNAP, and yeast SNAREs with yeast homologues of NSF and SNAP proteins. This observation led to the hypothesis that, despite low sequence homology, SNARE proteins are structurally similar among eukaryotes. SNARE proteins can be classified into two groups depending on whether they interact with SNARE binding partners via conserved glutamine (Q-SNAREs) or arginine (R-SNAREs). Much of the published structural data available is for SNAREs involved in exocytosis (either in yeast or synaptic vesicles). This paper describes circular dichroism, Fourier transform infrared spectroscopy, and dynamic light scattering data for a set of yeast v- and t-SNARE proteins, Vti1p and Pep12p, that are Q-SNAREs involved in intracellular trafficking. Our results suggest that the secondary structure of Vti1p is highly α-helical and that Vti1p forms multimers under a variety of solution conditions. In these respects, Vti1p appears to be distinct from R-SNARE proteins characterized previously. The α-helicity of Vti1p is similar to that of Q-SNARE proteins characterized previously. Pep12p, a Q-SNARE, is highly α-helical. It is distinct from other Q-SNAREs in that it forms dimers under many of the solution conditions tested in our experiments. The results presented in this paper are among the first to suggest heterogeneity in the functioning of SNARE complexes.