Resistance to atrazine (a photosystem II [PSII] inhibitor) is prevalent in waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] across the U.S. Midwest. Previous research suggests that target-site mutation or rapid metabolism of atrazine mediated by glutathione S-transferase (GST) conjugation confers resistance in A. tuberculatus from Illinois. The distribution and mechanism of resistance to atrazine in A. tuberculatus populations from Nebraska (NE) are unknown. In this research we (1) evaluated the response and frequency of resistance in NE A. tuberculatus to soil-applied PSII (metribuzin and atrazine) and protoporphyrinogen oxidase (sulfentrazone) inhibitors, as well as POST-applied atrazine; and (2) determined the mechanism of atrazine resistance in NE A. tuberculatus. The chloroplastic psbA gene, coding for a D1 protein (the target site of atrazine) was sequenced in 85 plants representing 27 populations of A. tuberculatus. Furthermore, 24 plants selected randomly from four atrazine-resistant (AR) populations were used to determine the metabolism of atrazine via GST conjugation. Results from the soil-applied herbicide evaluation suggest that metribuzin (0.56 kg ai ha−1) and sulfentrazone (0.28 kg ai ha−1) were effective on A. tuberculatus management. PRE and POST screenings against atrazine in the greenhouse indicate that atrazine (1.345 kg ai ha−1) was not effective on 39% and 73% of the A. tuberculatus populations evaluated (total of 109 and 85 populations, respectively), suggesting the prevalence of atrazine resistance in A. tuberculatus in NE. Sequence analysis of the psbA gene found no known point mutations conferring atrazine resistance. However, the AR plants conjugated atrazine via GST activity faster than the known atrazine-susceptible A. tuberculatus. Overall, the outcome of this study demonstrates the predominance of metabolism-based resistance to atrazine in A. tuberculatus from NE, which may predispose this species to evolve resistance to other herbicides. The use of integrated management strategies for A. tuberculatus is crucial for the control of this troublesome species.

Experiments were conducted at three locations in Louisiana from 1992 to 1994 to evaluate broadleaf weed control with sulfentrazone. Sulfentrazone at 0.42 kg ai/ha applied PPI or PRE provided at least 93% control of entireleaf morningglory in all years at all locations, which was greater than control with metribuzin PRE. Prickly sida control with sulfentrazone ranged from 83 to 94%, which was equal to or greater than control with metribuzin. Hemp sesbania and sicklepod control with sulfentrazone was unacceptable (≤75%) regardless of application method and generally was lower than control with metribuzin. Greater than 90% control of smellmelon and hophornbeam copperleaf was observed with all treatments. Minor but transient soybean injury was noted at one location in one year. Soybean yields following sulfentrazone PRE at St. Joseph were greater than yields with metribuzin as a result of the general increase in broadleaf weed control.

Tactics that target seedbanks are important components of weed management systems; however, such tactics can be difficult to adopt because consequences of seedbank reduction are often unclear. This study developed model-based software to provide insights on the economic outcomes, in the context of chile pepper production, of additions to tall morningglory seedbanks. Data for the model were derived from this and previous studies. In this study, field experiments were conducted to determine chile pepper yield and harvest efficiency responses to mid-season tall morningglory infestations. The experimental treatments were factorial combinations of herbicide (pendimethalin-treated, nontreated) and tall morningglory density (0, 4, 8, 12, 16, 20 plants 10-m row–1). Treatments were installed 9.5 weeks after crop seeding. Data collected included fresh weight of marketable chile peppers and time required for one individual to harvest 10-m of crop row, which was used to calculate the amount of chile pepper harvested in 1 min (harvest efficiency). Results indicated that crop yield was not influenced by tall morningglory density, pendimethalin treatment and interactions between tall morningglory density and pendimethalin. Harvest efficiency was influenced by tall morningglory density but was not influenced by herbicide treatment or interactions between herbicide treatment and tall morningglory density. Each additional tall morningglory plant decreased the amount of chile pepper harvested in 1 min by 9.7 g. The results of this and previous studies were used to develop model-based software that presents tall morningglory seedbank density effects on: (1) tall morningglory seedling densities after pendimethalin, (2) time requirements for hand-hoeing after pendimethalin, and (3) time requirements for hand-harvesting to acquire yield goals. The model-based software is intended to be used in the instruction of weed seedbank management strategies. By presenting seedbank density effects on weed control outcomes and crop production expenses, the model-based software might promote adoption of seedbank reduction strategies.

Pendimethalin control failures on tall morningglory are critical shortcomings in weed-control programs for chile pepper in New Mexico. Using weed seedbank augmentation, we conducted a field study to (1) determine if pendimethalin control of tall morningglory is affected by tall morningglory seedbank density, and (2) identify weed community factors that influence labor for removing the tall morningglory plants that escape pendimethalin. The field study was complemented with a growth chamber study conducted to clarify the effects of pendimethalin rate on the putative association between tall morningglory seedbank density and pendimethalin control outcomes. Under field conditions and after square-root transformation of the dependent variable, the effects of seedbank density on seedling escape density were described with natural logarithmic functions. Although pendimethalin control of tall morningglory decreased with increasing seedbank density, seedbank additions increased labor requirements for removing tall morningglory at only a site-year characterized by low population densities in the indigenous weed community. In growth chambers, increasing pendimethalin rate negatively influenced the effects of increasing seedbank density on pendimethalin control failures. This study shows that pendimethalin control of tall morningglory is reduced when seedbank densities of this species are high. Knowledge of seedbank density effects on specific control outcomes may influence grower attitudes on management strategies that target weed seedbanks.

A study was established to evaluate weed control and crop response of imidazolinone-tolerant (IMI-tolerant) rice in water-seeded culture with imazethapyr at 70, 105, and 140 g ai/ha at four different soil application timings with or without 70 g/ha imazethapyr postemergence (POST). Application timings included preplant incorporated (PPI), surface application prior to seeding (SURFACE), following seeding (SEED), and at pegging (PEG). The PEG treatments were applied when green leaf tissue had emerged from the seed and the root had begun to extend downward into the soil. Response of barnyardgrass, red rice, and rice injury was not affected by the addition of an imazethapyr POST application. Barnyardgrass control was above 90% at 28 d after treatment (DAT) for both years. At 42 DAT, barnyardgrass control decreased to 88% in 1998, compared with 94% control in 1999. Averaged over years, Indian jointvetch control ranged from 44 to 74% at 28 DAT and 41 to 71% at 42 DAT. Indian jointvetch control was inconsistent and lacked uniformity over all factors. Rice injury increased as application timing was delayed from PPI to PEG in 1998 at both rating dates. In 1999, injury was not observed with imazethapyr at 28 DAT, and 1% injury was observed at 42 DAT with all imazethapyr application timings.

Field studies were conducted in Alabama in 1998 and 1999 to evaluate fomesafen preemergence (PRE) in glyphosate-resistant cotton. Fomesafen (0.3 and 0.4 kg ai/ha), fluometuron (1.4 kg ai/ha), and pyrithiobac (0.05 kg ai/ha) were applied alone or in tank mixtures. Glyphosate (0.4 kg ae/ha) was applied postemergence over-the-top (POT) and postemergence directed (PD). Regardless of PRE treatment, POT followed by (fb) PD applications of glyphosate were necessary for greater than 82% sicklepod control at midseason. In the absence of glyphosate, fomesafen, and fomesafen-containing tank mixtures controlled common cocklebur and Ipomoea species 77 and 72%, respectively, 14 d after PD application. However, two applications of glyphosate were needed for >94% season-long control of common cocklebur and Ipomoea species because of continued germination throughout the growing season. Postemergence applications of glyphosate added a 1,000 kg/ha seed cotton yield increase to all PRE treatments at both locations. Common cocklebur, Ipomoea species, and sicklepod control was not significantly increased by the addition of fomesafen PRE tank mixtures fb glyphosate postemergence as compared with glyphosate postemergence only.

A study was conducted in 1999 to evaluate weed control and imidazolinone-tolerant (IMI-tolerant) rice response to 140 g ai/ha imazethapyr at different application timings and to flood depths of 5, 10, and 20 cm at the Rice Research Station near Crowley, LA, and at the Northeast Research Station near St. Joseph, LA. Barnyardgrass control, rice injury, and rice yield were not influenced by flood depth; therefore, data were averaged over flood depths. Season-long barnyardgrass control was greater than 80% at both locations with imazethapyr applied preplant incorporated (PPI), early postemergence (EPOST), and mid-postemergence (MPOST). IMI-tolerant rice injury at 7 d after late postemergence (LPOST) treatment (DAT) was less than 5% for all imazethapyr treatments at Crowley and 1 to 8% for PPI, preemergence (PRE), delayed preemergence (DPRE), and EPOST timings at St. Joseph. However, IMI-tolerant rice injury was 5% or less at 42 DAT for all treatments at Crowley and St. Joseph. IMI-tolerant rice yield at Crowley was 2,780 to 3,000 kg/ha following applications of imazethapyr at PPI, PRE, DPRE, and EPOST, and these yields were higher than the yields of the MPOST and LPOST treatments. At St. Joseph, IMI-tolerant rice yields were equal for imazethapyr applied PPI, DPRE, and EPOST treatments compared with Crowley.