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1 results

  • Chapter 6 - Computer-Aided Sperm Analysis

  • David Mortimer, Lars Björndahl, Karolinska Institutet, Stockholm, Christopher L. R. Barratt, University of Dundee, José Antonio Castilla, Roelof Menkveld, Stellenbosch University, South Africa, Ulrik Kvist, Karolinska Institutet, Stockholm, Juan G. Alvarez, Trine B. Haugen
  • Book:
    A Practical Guide to Basic Laboratory Andrology
    Published online:
    16 February 2022
    Print publication:
    10 March 2022, pp 130-154

  • Summary

    Discusses the development, application and limitations of computer-aided sperm analysis (CASA) methods, including the deriation of kinematic measures of human sperm motility. Explains the technical and biological factors that limit CASA's functionality for human semen analysis and summarizes expert recommendations on the use of CASA for human semen analysis and sperm kinematics analysis (including sperm-mucus penetration and sperm hyperactivation). Issues related to the non-comparability of different CASA systems are considered, along with quality control for CASA. A strategy for validating a CASA system for human semen analysis, based on expectations of accuracy and precision, is also provided. Finally the use of CASA for analyzing sperm function tests, and new and future CASA technology (including the application of artificial intelligence technqiues) are surveyed.