Showy crotalaria (Crotalaria spectabilis Roth) seed dormancy was due to seed coat impermeability to water. The seed coats became impermeable during later stages of maturation on the plant. When incubated immediately after harvest, 9% of the mature, black seeds (11% water content) imbibed water and germinated. The remaining 91% had impermeable coats and did not germinate. After dry storage for 3 months at 23 C, 24% of the seeds imbibed water and germinated. Seeds of another seed lot, which contained seeds of different maturity stages, attained 47% imbibition and germination after storage for 1 yr. The dry, less mature green seeds had a higher percentage (51%) of permeable seeds than did the black seeds (31%) from the same lot. Several seed coat treatments induced imbibition of water and germination of previously impermeable seeds. Effective treatments included piercing of seed coats, scarification with sandpaper, soaking in 70 C water, and simply applying pressure on the strophiole area. Covering the pressed strophiole with petroleum jelly significantly blocked imbibition and indicated that water entered the seeds at only the pressed area. However, studies indicated natural loss of impermeability in showy crotalaria seeds may occur at other sites as well.

The seed coat of Trifolium repens L. was studied with special emphasis on the development of the strophiole, which is the site for water entry during imbibition in leguminous seeds. The epidermal cells of the strophiole are longer than the cells in the remainder of the seed epidermis in the mature ovule. During seed development the median cells of the strophiolar epidermis divide periclinally into an outer layer of palisade cells and an inner layer of isodiametric cells. Prior to maturity a fissure is formed between some of the palisade cells in the centre of the strophiole. It is suggested that tension develops between the palisade cells and the iso-diametrical cells during later maturation stages causing the formation of the fissure which it is believed functions in water uptake. It is indicated that the ‘light line’ is caused by alteration of cellulose microfibrillar orientation in palisade cell walls. It is confirmed that removal of the epicuticular wax from hard seeds by rinsing in absolute alcohol or hexane does not induce water imbibition. Only when seed coats are mechanically abraded do hard seeds germinate.