With recent increased focus on positive welfare in animal welfare science, there is demand for objective positive welfare indicators. It is unclear whether changes in body surface temperature can be used to non-invasively identify and quantify positive states in mammals. We recorded continuous measurements of tail surface temperature using infra-red thermography (IRT) and concurrent behavioural observations in male and female Wistar rats (Rattus norvegicus). If tail surface temperature can differentiate between positive and negative experiences, we expect a qualitatively different response compared to negative experiences. Three groups of rats were presented with increasing magnitudes of food rewards (neutral/none, one and three rewards). The rats were placed in an arena to which they were habituated and filmed for 30 s before and 30 min after exposure to different rewards. Tail temperature initially decreased from the pre-reward baseline and subsequently returned towards baseline temperature. The overall pattern of the change was the same as for rats subjected to negative stimuli in previous studies. Nevertheless, dynamic changes in tail temperature, specifically the rate of recovery and the behavioural response (exploration), differed between neutral and rewarded rats but failed to distinguish reward magnitude. Sex differences were found in both thermal and behavioural responses, unrelated to reward magnitudes. Female rats exhibited a greater initial response with a slower recovery than male rats, emphasising the value of using of both sexes in animal welfare research. This study improves our understanding of the effects of positive emotions induced by food reward on peripheral body temperature and behaviour.

The goal of this work was the assessment of commercial vineyard water status using on-the-go thermal imaging. On-the-go thermal imaging acquisition was conducted with a thermal camera operating at 1.20 m distance from the canopy, mounted on a quad moving at 5 km/h. Canopy temperature, cross water stress index (CWSI) and stomatal conductance index (Ig) were strongly and significantly correlated to stem water potential (Ψstem) in east and west side of the canopy. For CWSI, the values of the coefficient of determination (R2) were 0.88*** and 0.73*** for east and west sides, respectively. As regards the index Ig, its relationships with Ψstem showed R2=0.89*** and R2=0.77*** for east and west sides, respectively. These results are promising and evidence the potential of on-the-go thermal imaging to become a new tool to evaluate the vineyard water status.

To use VRI systems, a field is divided into irrigation management zones (IMZs). While IMZs are dynamic in nature, most of IMZs prescription maps are static. High-resolution thermal images (TI) coupled with measured atmospheric conditions have been utilized to map the within-field water status variability and to delineate in-season IMZs. Unfortunately, spaceborne TIs have coarse spatial resolution and aerial platforms require substantial financial investments, which may inhibit their large-scale adoption. Three approaches are proposed to facilitate large-scale adoption of TI-based IMZs: 1) increase of the capacity of aerial TI by enhancing their spatial resolution; 2) sharpening the spatial resolution of satellite TI by fusing satellite multi-spectral images in the visible-near-infrared (VIS-NIR) range; 3) increase the capacity of aerial TI by fusing satellite multi-spectral images in the VIS-NIR range. The scientific and engineering basis of each of the approaches is described together with initial results.