A laboratory study was conducted to determine the effects of two adjuvants and temperatures at time of treatment on efficacy, absorption, and translocation of thidiazuron defoliant on cotton. Five days after treatment at 30/21 C day/night temperatures, leaf drop was 17% with no adjuvant, 37% with addition of crop oil concentrate, 40% with ammonium sulfate, and 75% with two adjuvants combined. At 21/13 C day/night temperatures, there was less than 10% leaf drop with all treatments. At 10 d after treatment, leaf drop was not different among treatments at the high or low temperatures. Shoot regrowth at high and low temperature was reduced 55 to 60% with addition of both adjuvants and 44 to 50% with each adjuvant or with no adjuvant when compared to plants defoliated by hand. Absorption of 14C-thidiazuron was not affected by variations in temperature during the time of treatment but was affected by adjuvants. With no adjuvants, absorption was 7 to 10%. With 1.25% by vol crop oil concentrate, absorption was 33 to 46%. Addition of ammonium sulfate resulted in 18 to 19% absorption, and the combination of ammonium sulfate and crop oil concentrate increased absorption to 65 to 68%. There was no movement of radiolabel away from treated leaves as determined by autoradiographs of treated plants.

Seed germination of the hemiparasitic angiosperm Striga hermonthica is elicited by compounds present in the root exudates of the host plant. A variety of chemicals can substitute for the host-derived signal although the exact mechanism through which these act is unknown. In the present study, thidiazuron (TDZ), a cytokinin-active urea derivative was found to stimulate germination. This thidiazuron-induced germination was shown to be mediated by endogenous ethylene, by a similar mechanism to germination induced by host-root exudate and the synthetic stimulant GR-24. Comparing the effects of TDZ, host-root exudate, GR-24 and the ethylene precursor 1-aminocyclopropane-1-car-boxylic (ACC) suggests that ethylene production increases as a result of increased ACC synthesis and is consistent with a model for Striga seed germination in which host-derived signals and other stimulants act by eliciting the synthesis of ethylene via a stimulation of ACC synthase activity. All germination stimulants tested trigger a rapid increase in O2 uptake by conditioned seeds. The patterns of O2 uptake following TDZ or GR-24 treatments show similarities and three distinct phases of respiration are apparent. In contrast, stimulation of O2 uptake by ethylene results in a different pattern, with no distinct phases obvious following the initial burst of activity.