Many wildlife rescues have been carried out over the past few decades but most were not documented and were controversial. However, such operations can be useful, providing risks are evaluated and elementary guidelines are followed. In addition to saving lives and being desirable from an ethical point of view, such operations can be very valuable for both biological research and conservation, and are certainly preferable than the alternative: to do nothing. Previous rescues are reviewed and the operation conducted in French Guiana during the filling of the Petit Saut reservoir is described. Objectives, methods, results and financial aspects are discussed. Around 5500 mammals and reptiles were captured, sampled and translocated to a protected area. The successful results of the translocation, the large amount of original data obtained, the interest shown by scientists in our samples and the subsequent new conservation impetus that appeared in the government corroborate our initial conviction that the operation was worthwhile.