Available pieces of information on Cicadulina sp. leafhopper biology, with specific references to the suitability of various species for mass rearing and resistance screening for maize streak virus (MSV) are critically reviewed. The following are summarized: (a) effect of distribution pattern and species composition on the efficiency of collection of live specimens for establishing a colony for mass rearing; (b) species biology and fecundity as affected by temperature and humidity; (c) species specificity in MSV transmission; (d) host plant preference and adaptation to mass rearing conditions. All these parameters affect the suitability of various Cicadulina species used as vectors in maize resistance screening and breeding to MSV disease.