The amino acid compositions of whole body tissue of European seabass, gilthead seabream and turbot of two different size classes were determined. No significant differences were detected between species and the composition was not affected by body size. Based on whole body indispensable amino acids (IAA) to total IAA ratios (A/E ratios), the IAA requirement profiles for the three species were estimated and compared to data available to date for some amino acids, obtained using dose-response curves.