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yeast two-hybrid system

Chinese Journal of Agricultural Biotechnology (1)

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1 results

Identification of oxalate oxidase in rice defence system against rice blast
Feng Jie, Takano Makoto
Journal:

Chinese Journal of Agricultural Biotechnology / Volume 1 / Issue 3 / December 2004

Published online by Cambridge University Press:

12 February 2007, pp. 167-171

Print publication:

December 2004
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A rice cDNA library was screened using OSK3 protein kinase as bait in a yeast two-hybrid system. The gene encoding oxalate oxidase was one of the positive clones interacting with OSK3 protein kinase. The interactions were verified by detecting expression of the reporter gene lacZ. The results suggest that oxalate oxidase is a downstream element in the disease resistance signal cascade mediated by OSK3 protein kinase in rice.