Trophy hunting (TH) is a form of wildlife use in which individuals pay to hunt under regulated conditions and retain selected body parts as trophies. Tanzania permits TH in areas with different land-use designations, covering diverse habitats. However, human activities such as settlement, agriculture and livestock grazing can threaten habitats and wildlife populations, particularly as revenue from TH declines. Using satellite data from between 2013 and 2023, we quantified changes in functional area and land cover across 15 hunting blocks in northern Tanzania. Over this period, functional area in hunting blocks decreased by c. 22.4% in Game Controlled Areas (GCAs) and by c. 18.0% in Open Areas (OAs), attributed to human encroachment. Longido North GCA experienced the most substantial land-cover change, with a 76% increase in dense vegetation. An analysis comparing the 2013–2019 and 2019–2023 data revealed consistent shrinkages in grassland and mixed and dense vegetation within the Longido North GCA, Masai OA and Irkishbor OA hunting blocks. Although individual land-cover losses in the given periods remained below 50% (our threshold for major habitat loss), these cumulative changes reduce habitat suitability, posing risks to biodiversity and the sustainability of TH. This study reveals the extent of human pressures on hunting blocks and calls for integrated regional planning, active management and targeted conservation strategies.