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9 - Cementochronology for Archaeologists: Experiments and Testing for an Optimized Thin-Section Preparation Protocol

from Part II - Protocols

Published online by Cambridge University Press:  20 January 2022

By
Eric Pubert ,
Stephan Naji ,
Lionel Gourichon ,
Frédéric Santos  and
William Rendu
Edited by
Stephan Naji ,
William Rendu  and
Lionel Gourichon
Stephan Naji
Affiliation:
New York University
William Rendu
Affiliation:
University of Bordeaux (CNRS)
Lionel Gourichon
Affiliation:
Université de Nice, Sophia Antipolis
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Summary

For human dental cementum research, sample preparation protocol is now widely tested, validated, and standardized, thanks to the low variability in teeth morphology. For non-human mammals, posterior teeth are typically preferred. However, the taxa diversity implies a significant variation in morphology or specific characteristics for certain species (equids, suids), leading to multiple unstandardized protocols. This work aims to improve protocols for producing a thin section by optimizing the parameters, minimizing the risk of errors, and offering an easily reproducible quality of thin-sections. The result of 26 experiments and 124 analyses during stages of consolidation (embedding), cutting, gluing, and finishing (grinding) allowed the co-authors' combined experience from multiple laboratories to propose standardized humans and ungulates (large teeth) protocols for the systematic analysis of dental research collections.

Keywords

Cementumprotocolstandardizationhistologyungulatehumanembeddingthin-section preparation
Type
Chapter
Information
Dental Cementum in Anthropology , pp. 173 - 188
Publisher: Cambridge University Press
Print publication year: 2022

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References

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