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7 - Cryopreservation of surgically retrieved sperm

from Cryopreservation of sperm and testicular tissue

Published online by Cambridge University Press:  06 July 2010

By
Peter T. K. Chan
Edited by
Ri-Cheng Chian  and
Patrick Quinn
Ri-Cheng Chian
Affiliation:
McGill University, Montréal
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Summary

This chapter focuses on various issues in the cryopreservation of surgically retrieved sperm. When the male partner is unable to produce an adequate amount of ejaculated sperm with good quality for intracytoplasmic sperm injection (ICSI), surgical sperm retrieval is indicated. Various techniques have been described and modified for sperm retrieval since the introduction of ICSI. The three principal sources of sperm are epididymides, testes, and ejaculation. The quantity of sperm retrievable surgically from the epididymides and testes is generally small compared with the amount of ejaculated sperm. With epididymal sperm, there are reductions in the concentration of viable spermatozoa as well as a significant decrease (by 50%) in the percentage of motile spermatozoa. For men with azoospermia, cryopreservation of sperm retrieved surgically in advance of the fertilization stage can allow better planning for the ICSI cycle and minimize unnecessary ovarian stimulation.
Type
Chapter
Information
Fertility Cryopreservation , pp. 51 - 56
Publisher: Cambridge University Press
Print publication year: 2010

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