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Chapter 33 - Semen analysis

Published online by Cambridge University Press:  19 May 2010

By
Susan A. Rothmann  and
Angela A. Reese
Edited by
Larry I. Lipshultz ,
Stuart S. Howards  and
Craig S. Niederberger
Larry I. Lipshultz
Affiliation:
Baylor College of Medicine
Stuart S. Howards
Affiliation:
University of Virginia
Craig S. Niederberger
Affiliation:
University of Illinois, Chicago
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Summary

Semen specimens usually are collected by masturbation into a wide-mouthed polypropylene container from a batch or lot tested for lack of sperm toxicity. Mixing a semen sample thoroughly is critical for accurate sperm counts, both initially and throughout each step of semen analysis. Sperm viability testing typically uses a nuclear exclusion stain to determine whether non-motile sperm are alive and not able to move. The component of sperm morphology is one of the most predictive measures of fertility potential and therapeutic outcome. The semen should be examined microscopically for the presence of bacteria, round cells, debris, agglutination, or aggregation. The influence of the female partner in human fertility plays a huge confounding role in both defining fertility and interpreting the association of specific semen analysis measures with fertility. Sperm motility quality controls are available in two formats: frozen aliquots of semen or video recordings on CD-ROM, tape, or digital file.

Keywords

abnormal accessory gland functionejaculatory dysfunctionneural control of ejaculationurethraspinal cord injurysperm motilitypenile vibratory stimulationretroperitoneal lymph node dissection
Type
Chapter
Information
Infertility in the Male , pp. 550 - 573
Publisher: Cambridge University Press
Print publication year: 2009

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