Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-13T03:32:53.518Z Has data issue: false hasContentIssue false

Chapter 35 - Zygote Cryopreservation for IVF

from Section 7 - Embryo Cryopreservation

Published online by Cambridge University Press:  07 August 2023

By
Dean E. Morbeck
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
Get access

Summary

This chapter will discuss the techniques for fertilization verification for oocytes fertilized both conventionally and by intracytoplasmic sperm injection (ICSI). The formation of the pronuclei is the first indication of the fusion of male and female gametes. Practical considerations and laboratory protocols are detailed in the chapter.

Keywords

2PNcryopreservationzygote
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 255 - 262
Publisher: Cambridge University Press
Print publication year: 2023

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Testart, J., Lassalle, B., Belaisch-Allart, J., et al. High pregnancy rate after early human embryo freezing. Fertil Steril 1986; 46:268–72.CrossRefGoogle ScholarPubMed
Demoulin, A., Jouan, C., Gerday, C. and Dubois, M. Pregnancy rates after transfer of embryos obtained from different stimulation protocols and frozen at either pronucleate or multicellular stages. Hum Reprod 1991; 6:799–804.CrossRefGoogle ScholarPubMed
Veeck, L. L., Amundson, C. H., Brothman, L. J., et al. Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. Fertil Steril 1993; 59:1202–7.CrossRefGoogle ScholarPubMed
Damario, M., Hammitt, D., Session, D. and Dumesic, D. Embryo cryopreservation at the pronuclear stage and efficient embryo use optimizes the chance for a liveborn infant from a single oocyte retrieval. Fertil Steril 2000; 73:767–73.CrossRefGoogle ScholarPubMed
Damario, M. A., Hammitt, D. G., Galanits, T. M., Session, D. R. and Dumesic, D. A. Pronuclear stage cryopreservation after intracytoplasmic sperm injection and conventional IVF: implications for timing of the freeze. Fertil Steril 1999; 72:1049–54.CrossRefGoogle ScholarPubMed
Edgar, D. H. and Gook, D. A. A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update 2012; 18:536–54.CrossRefGoogle ScholarPubMed
Vanderzwalmen, P., Zech, N. H., Ectors, F. et al. Blastocyst transfer after aseptic vitrification of zygotes: an approach to overcome an impaired uterine environment. Reprod Biomed Online. 2012; 25(6):591–9.CrossRefGoogle ScholarPubMed
Parmegiani, L., Beilby, K. H., Arnone, A. et al. Testing the efficacy and efficiency of a single “universal warming protocol” for vitrified human embryos: prospective randomized controlled trial and retrospective longitudinal cohort study. J Assist Reprod Genet. 2018; 35:1887–95.Google Scholar
Glujovsky, D., Blake, D., Farquhar, C. and Bardach, A. Cleavage stage versus blastocyst stage embryo transfer in assisted reproductive technology. Cochrane Database Syst Rev 2012; 7:CD002118.Google Scholar
Morbeck, D. E. Importance of supply integrity for in vitro fertilization and embryo culture. Semin Reprod Med 2012; 30:182–90.Google ScholarPubMed
Wolff, H. S., Fredrickson, J. R., Walker, D. L. and Morbeck, D. E. Advances in quality control: mouse embryo morphokinetics are sensitive markers of in vitro stress. Hum Reprod 2013; 28:1776–82.CrossRefGoogle ScholarPubMed
Shapiro, B. S., Daneshmand, S. T., Garner, F. C., et al. Similar ongoing pregnancy rates after blastocyst transfer in fresh donor cycles and autologous cycles using cryopreserved bipronuclear oocytes suggest similar viability of transferred blastocysts. Fertil Steril 2010; 93:319–21.CrossRefGoogle ScholarPubMed
Wright, G., Wiker, S., Elsner, C., et al. Observations on the morphology of pronuclei and nucleoli in human zygotes and implications for cryopreservation. Hum Reprod 1990; 5:109–15.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×