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Urinary excretion of purine derivatives and tissue xanthine oxidase (EC 1.2.3.2) activity in buffaloes (Bubalis bubalis) with special reference to differences between buffaloes and Bos taurus cattle

Published online by Cambridge University Press:  09 March 2007

X. B. Chen ,
L. Samaraweera ,
D. J. Kyle ,
E. R. Ørskov  and
H. Abeygunawardene
X. B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
L. Samaraweera
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. J. Kyle
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
H. Abeygunawardene
Affiliation:
Faculty of Veterinary Medicine, Peradeniya University, Peradeniya, Sri Lanka
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Abstract

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The urinary excretion of purine derivatives (PD) was measured in six buffaloes (Bubalis bubalis) during fasting and in fourteen buffaloes given four restricted levels of roughage (2·5-4·8 kg DM/d). Only allantoin and uric acid, not xanthine and hypoxanthine, were present in the urine, the pattern of excretion being similar to that in cattle. The fasting PD excretion amounted to 0·20 (SD 0·06) mmol/kg metabolic weight (W0·75) per d, and the rate of PD excretion as a linear function of feed intake was 5·2 mmol/kg digestible organic matter intake. Both values were considerably lower than the values for cattle reported in the literature. Creatinine excretion values were 0·33 (SD 0·06) and 0·44 (SD 0·09) mmol/kg W0.75 per d determined in fasting and feeding periods respectively. Fasting N excretion was 257 (SD 49) mg N/kg W0.75 per d. Both creatinine and fasting N excretions were also lower than in cattle. The activities of xanthine oxidase (EC 1.2.3.2) in plasma, liver and intestinal mucosa were determined in buffaloes, cattle and sheep. Xanthine oxidase activities in buffaloes were 24·5 (SD 2·7) unit/l plasma and 0·44 (SD 0·02) and 0·31 (SD 0·10) unit/g fresh tissue in liver and intestinal mucosa respectively. These activities were higher than those in cattle and sheep. Xanthine oxidase was practically absent from plasma and intestine of sheep. It is suggested that the differences in PD excretion between buffaloes and cattle were probably due to the smaller proportion of plasma PD that was disposed of in the urine of buffaloes.

Keywords

BuffaloPurine derivativesXanthine oxidase
Type
purine metabolism in buffaloes
Information
British Journal of Nutrition , Volume 75 , Issue 3 , March 1996 , pp. 397 - 407
Copyright
Copyright © The Nutrition Society 1996

References

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