Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-28T04:34:50.844Z Has data issue: false hasContentIssue false
  • English
  • Français

Urinary excretion of purine derivatives and tissue xanthine oxidase (EC 1.2.3.2) activity in buffaloes (Bubalis bubalis) with special reference to differences between buffaloes and Bos taurus cattle

Published online by Cambridge University Press:  09 March 2007

X. B. Chen ,
L. Samaraweera ,
D. J. Kyle ,
E. R. Ørskov  and
H. Abeygunawardene
X. B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
L. Samaraweera
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. J. Kyle
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
H. Abeygunawardene
Affiliation:
Faculty of Veterinary Medicine, Peradeniya University, Peradeniya, Sri Lanka
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The urinary excretion of purine derivatives (PD) was measured in six buffaloes (Bubalis bubalis) during fasting and in fourteen buffaloes given four restricted levels of roughage (2·5-4·8 kg DM/d). Only allantoin and uric acid, not xanthine and hypoxanthine, were present in the urine, the pattern of excretion being similar to that in cattle. The fasting PD excretion amounted to 0·20 (SD 0·06) mmol/kg metabolic weight (W0·75) per d, and the rate of PD excretion as a linear function of feed intake was 5·2 mmol/kg digestible organic matter intake. Both values were considerably lower than the values for cattle reported in the literature. Creatinine excretion values were 0·33 (SD 0·06) and 0·44 (SD 0·09) mmol/kg W0.75 per d determined in fasting and feeding periods respectively. Fasting N excretion was 257 (SD 49) mg N/kg W0.75 per d. Both creatinine and fasting N excretions were also lower than in cattle. The activities of xanthine oxidase (EC 1.2.3.2) in plasma, liver and intestinal mucosa were determined in buffaloes, cattle and sheep. Xanthine oxidase activities in buffaloes were 24·5 (SD 2·7) unit/l plasma and 0·44 (SD 0·02) and 0·31 (SD 0·10) unit/g fresh tissue in liver and intestinal mucosa respectively. These activities were higher than those in cattle and sheep. Xanthine oxidase was practically absent from plasma and intestine of sheep. It is suggested that the differences in PD excretion between buffaloes and cattle were probably due to the smaller proportion of plasma PD that was disposed of in the urine of buffaloes.

Keywords

BuffaloPurine derivativesXanthine oxidase
Type
purine metabolism in buffaloes
Information
British Journal of Nutrition , Volume 75 , Issue 3 , March 1996 , pp. 397 - 407
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Agricultural Research Council (1984). The Nutrient Requirements of Ruminant Livestock, Supplement no. 1. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Al-Khalidi, U. A. S. & Chaglassian, T. H. (1965). The species distribution of xanthine oxidase. Biochemical Journal 97, 318–320.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (1965). Oficial Methods of Analysis of the Association of Official Analytical Chemists, 10th ed., pp. 327–328. Washington, DC: AOAC.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. & Gasa, J. (1991). Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116, 309–317.CrossRefGoogle Scholar
Balcells, J., Parker, D. S. & Seal, C. J. (1992). Purine metabolite concentrations in portal and peripheral blood of steers, sheep and rats. Comparative Biochemistry and Physiology 101B, 633–636.Google Scholar
Chen, X. B., Grubic, G., Ørskov, E. R. & Osuji, P. (1992). Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers. Animal Production 55, 185–191.Google Scholar
Chen, X. B., Hovell, F. D. DeB. & ørskov, E. R. (1990 a). Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut. British Journal of Nutrition 63, 197–205.CrossRefGoogle ScholarPubMed
Chen, X. B., Kyle, D. & Ørskov, E. R. (1993). Measurement of allantoin in urine and plasma by high-performance liquid chromatography with pre-column derivatization. Journal of Chromatography 617, 241–247.CrossRefGoogle ScholarPubMed
Chen, X. B., Mathieson, J., Hovell, F. D. DeB. & Reeds, P. J. (1990 b). Measurement of purine derivatives in urine of ruminants using automated methods. Journal of the Science of Food and Agriculture 53, 23–33.CrossRefGoogle Scholar
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1990 c). Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition 63, 121–129.CrossRefGoogle ScholarPubMed
Chen, X. B., Ørskov, E. R. & Hovell, F. D. DeB. (1991). The use of intragastric infusion in studies on excretion of purine derivatives as a measure of microbial protein supply in ruminants. In Protein Metabolism and Nutrition. Proceedings of the 6th International Symposium on Protein Metabolism and Nutrition, vol. 2, pp. 67–70 [Eggum, B. 0., Boisen, S., Bsrsting, C., Danfter, A. and Hvelplund, T., editors]. Foulum, Denmark: National Institute of Animal Science.Google Scholar
Daniels, Z. M. (1993). Purine derivatives in urine and plasma of lactating cows given different levels of feed intake. MSc Thesis, Aberdeen University.Google Scholar
Dewhurst, R. J. & Webster, A. J. F. (1992). Effects of diet, level of intake, sodium bicarbonate and monensin on urinary allantoin excretion in sheep. British Journal of Nutrition 67, 345–353.CrossRefGoogle ScholarPubMed
Furth-Walker, D. & Amy, N. K. (1987). Regulation of xanthine oxidase activity and immunologically detectable protein in rats in response to dietary protein and iron. Journal of Nutrition 117, 1697–1703.CrossRefGoogle Scholar
Giesecke, D., Balsliemke, J., Suderum, K.-H. & Stangassinger, M. (1993). Plasma level, clearance and renal excretion of endogenous and ruminal purines in the bovine. Journal of Animal Physiology and Animal Nutrition 70, 180–189.CrossRefGoogle Scholar
Gupta, B. S., Bhargwa, V. N., Raina, N. N. & Singh, S. N. (1966). Studies on endogenous and metabolic faecal nitrogen in buffaloes. Indian Journal of Veterinary Science 36, 90–95.Google Scholar
Hashimoto, S. (1974). A new spectrophotometric assay method of xanthine oxidase in crude tissue homogenate. Analytical Biochemistry 62, 426–435.CrossRefGoogle ScholarPubMed
Larsen, K. (1972). Creatinine assay by reaction-kinetic principle. Clinica Chimica Acta 41, 209–217.CrossRefGoogle ScholarPubMed
Lawes Agricultural Trust (1983). Genstat 4 User Manual. Harpenden: Rothamsted Experimental Station.Google Scholar
Liang, J. B., Matsumoto, M., Ishida, M. & Young, B. A. (1993). Urinary allantoinexcretion in Malaysian cattle and buffaloes. In Proceedings of 7th World Conference on Animal Production, pp. 58–59. Alberta, Canada: University of Alberta.Google Scholar
Lindberg, J. E. (1989). Nitrogen metabolism and urinary excretion of purines in goat kids. British Journal of Nutrition 61, 309–321.CrossRefGoogle ScholarPubMed
Lindberg, J. E. & Murphy, M. (1991). Rumen metabolism, excretion of purine derivatives and milk composition in dairy cows fed rations with negative and positive protein balance values in the rumen. In Protein Metabolism and Nutrition. Proceedings of the 6th International Symposium on Protein Metabolism and Nutrition, vol. 2, pp. 336–337 [Eggum, B. O., Boisen, S., Barsting, C.Danfer, A and Hvelplund, T., editors]. Foulum, Denmark: National Institute of Animal Science.Google Scholar
Maloiy, G. M. O., Kay, R. N. B., Goodall, E. D. & Topps, J. H. (1970). Digestion and nitrogen metabolism in sheep and red deer given large or small quantities of water and protein. British Journal of Nutrition 24, 843–855.CrossRefGoogle Scholar
Moran, J. B. (1983). Aspects of nitrogen utilization in Asiatic water buffalo and Zebu cattle. Journal of Agricultural Science, Cambridge 100, 13–23.CrossRefGoogle Scholar
Mura, U., Osman, A. M., Mohamed, A. S. & Ipata, P. L. (1986). Studies on purine turnover in the camel (Camelus dromedarius) and Zebu (Bos indicus). Comparative Biochemistry and Physiology 84B, 589–593.Google Scholar
Muraoka, S. (1963). Studies on xanthine oxidase. 2. Biochimica et Biophysica Acta 73, 27–38.CrossRefGoogle Scholar
Norton, B. W., Moran, J. B. & Nolan, J. V. (1979). Nitrogen metabolism in Brahman cross, buffalo, Banteng and Shorthorn steers fed on low-quality roughage. Australian Journal of agricultural Research 30, 341–351.CrossRefGoogle Scholar
Ross, G. J. S. (1987). MLP User Manual. Rothamsted: Rothamsted Experimental Station.Google Scholar
Verbic, J., Chen, X. B., MacLeod, N. A. & Ørskov, E. R. (1990). Excretion of purine derivatives by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge 114, 243–248.CrossRefGoogle Scholar
Vercoe, J. E. (1976). Urinary allantoin excretion and digestible dry matter intake in cattle and buffalo. Journal of Agricultural Science, Cambridge 86, 613–615.CrossRefGoogle Scholar