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Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains

Published online by Cambridge University Press:  05 June 2009

J.D. Power ,
W. Harnett  and
T. Jenkins
J.D. Power
Affiliation:
School of Biological Sciences, University of Portsmouth, King Henry I Street, Portsmouth, Hants PO1 2DY, UK
W. Harnett
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK
T. Jenkins
Affiliation:
School of Biological Sciences, University of Portsmouth, King Henry I Street, Portsmouth, Hants PO1 2DY, UK
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Abstract

Surface iodination, extraction and SDS-PAGE analysis techniques were employed to characterize and compare the surface polypeptides of two strains of Strongyloides ratti. Third stage infective larvae and parasitic adults of homogonic and heterogonic strains were studied using a variety of surface labelling procedures and detergents for the extraction of labelled molecules. Profiles obtained from SDS-PAGE analysis demonstrated that homogonic and heterogonic strains of S. ratti have identical surface antigens.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 1 , March 1994 , pp. 57 - 62
Copyright
Copyright © Cambridge University Press 1994

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References

Bolton, A.F. & Hunter, W.M. (1973) The labelling of proteins to high specific radioactivities by conjugation to 125Iodine containing acylating agent; application to the radioimmunoassay. Biochemical Journal 133, 529–539.Google Scholar
Brindley, P.J., Gam, A.A., Pearce, E.J., Poindexter, R.W. & Neva, F.A. (1988) Antigens from the surface and excretions/secretions of the larva of Strongyloides stercoralis. Molecular and Biochemical Parasitology 28, 171–180.CrossRefGoogle ScholarPubMed
Fraker, P.J. & Speck, J.C. (1978) Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6- tetrachloro-3α, 6α-diphenyl glycoluril. Biochemical and Bio-physical Research Communications 80, 849–857.CrossRefGoogle Scholar
Garaté, T., Cabrera, Z., Copeman, D.B., Harnett, W., McLaren, D.J., Patterson, M. & Parkhouse, R.M.E. (1991) Surface antigens of male worms and microfilariae of Onchocerca gibsoni. International Journal for Parasitology 21, 37–45.CrossRefGoogle ScholarPubMed
Graham, G.L. (1938) Studies on Strongyloides II. Homogonic and heterogonic progeny of the single homogonically derived S. ratti parasite. American Journal of Hygiene 27, 221–234.Google Scholar
Grove, D.I. (1989) Strongyloidiasis; a major roundworm infection of man. London, New York, Philadelphia, Taylor & Francis Ltd.Google Scholar
Harnett, W. & Parkhouse, R.M.E. (1994) The nature and function of parasitic nematode surface and excretory-secretory antigens. in Sood, M.L. (Ed.) Perspectives in nematode physiology and biochemistry. In Press.Google Scholar
Hunter, W.M. & Greenwood, F.C. (1962) Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194, 495–496.Google Scholar
Laskey, R.A. & Mills, A.D. (1977) Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized films. FEBS Letters 82, 314–316.CrossRefGoogle Scholar
Maizels, R.M., Meghji, M. & Ogilvie, B.H. (1983) Restricted sets of parasite antigens from the surface of different stages and sexes of the nematode. Nippostrongylus brasiliensis. Immunology 48, 107–121.Google Scholar
Maizels, R.M., Sutanto, I., Gomez-Priego, A., Lilywhite, J. & Denham, D.A. (1985) Specificity of surface molecules of adult Brugia parasites; cross-reactivity with antibody from Wuchereria, Onchocerca and other human filarial infections. Tropical Medicine and Parasitology 36, 233–237.Google Scholar
Maizels, R.M., Burke, J., Sutanto, I., Purnomo, & Partono, F. (1986) Secreted and surface antigens from larval stages of Wuchereria bancrofti; the major human lymphatic parasite. Molecular and Biochemical Parasitology 19, 27–34.CrossRefGoogle ScholarPubMed
Maizels, R.M., Gregory, W.F., Kwan-Lim, G.E. & Selkirk, M.E. (1989) Filarial surface antigens: the major 29 kilodalton glycoprotein and a novel 17–200 kilodalton complex from adult Brugia malayi parasites. Molecular and Biochemical Parasitology 32, 213–228.CrossRefGoogle Scholar
Marshall, E. & Howells, R.E. (1985) An evaluation of different methods for labelling the surface of the filarial nematode, Brugia pahangi with 125Iodine. Molecular and Biochemical Parasitology 15, 295–304.CrossRefGoogle ScholarPubMed
Murrell, K.D., Graham, C.E. & McGreevy, M. (1983) Strongyloides ratti and Trichinella spiralis: net charge of epicuticle. Experimental Parasitology 55, 331–339.Google Scholar
Northern, C. & Grove, D. I. (1990) Strongyloides stercoralis: antigenic analysis of infective larvae and adult worms. International Journal for Parasitology 20, 381–387.Google Scholar
Northern, C., Grove, D.I., Warton, A. & Lovegrove, F.T. (1989) Surface labelling of Strongyloides ratti; stage-specificity and cross-reactivity with S. stercoralis. Clinical and Experimental Immunology 75, 487–492.Google Scholar
Parkhouse, R.M.E., Philipp, M. & Ogilvie, B.M. (1981) Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–352.CrossRefGoogle ScholarPubMed
Philipp, M. & Rumjaneck, F.D. (1984) Antigenic and dynamic properties of helminth surface structures. Molecular and Biochemical Parasitology 10, 245–268.Google Scholar
Philipp, M., Parkhouse, R.M.E. & Ogilvie, B.M. (1980) Changing proteins on the surface of a nematode parasite. Nature 287, 538–540.Google Scholar
Pritchard, D.I., Maizels, R.M., Behnke, J.M. & Appelby, P. (1984) Stage-specific antigens of Nematospiroides dubius. Immunology 53, 325–355.Google Scholar
Pritchard, D.I., Crawford, C.R., Duce, I.R. & Behnke, J.M. (1985) Antigen stripping from the nematode epicuticle using the cationic detergent cetyltrimethylammonium bromide (CTAB). Parasite Immunology 7, 575–585.Google Scholar