Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-10T15:52:56.607Z Has data issue: false hasContentIssue false
  • English
  • Français

Quantification of the energy required for the destruction of Balanus amphitrite larva by ultrasonic treatment

Published online by Cambridge University Press:  05 October 2010

Nishant Seth ,
Piyul Chakravarty ,
Lidita Khandeparker ,
Arga Chandrashekar Anil  and
Aniruddha B. Pandit
Nishant Seth
Affiliation:
Birla Institute of Technology and Science, Pilani, Rajasthan, India
Piyul Chakravarty
Affiliation:
Birla Institute of Technology and Science, Pilani, Rajasthan, India
Lidita Khandeparker
Affiliation:
Birla Institute of Technology and Science, Pilani, Rajasthan, India
Arga Chandrashekar Anil*
Affiliation:
National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa-403 004, India
Aniruddha B. Pandit
Affiliation:
Institute of Chemical Technology, N. P. Marg, Mumbai-400 019, India
*
Correspondence should be addressed to: A.C. Anil, National Institute of Oceanography, Council of Scientific and Industrial Research, Dona Paula, Goa-403 004, India email: acanil@nio.org
Get access Rights & Permissions [Opens in a new window]

Abstract

Ultrasonic treatment, a relatively less explored technology in water disinfection, was used to quantify the energy required for the destruction of larvae of barnacle Balanus amphitrite, which is a major marine fouling and a potential invasive organism. Since the power used and treatment time for disinfection are economically, and practically, the most important parameters, the energy required to pulverize the larvae into pieces ≤30 µm was determined as a function of the acoustic power density. The present investigation suggests that an ultrasonic system operating at 20 kHz and 0.0975 W/cm3 can effectively pulverize barnacle larvae having length (~440 µm) and breadth (~350 µm) within 45 seconds using 0.1 mJ/larva of pulverization energy. It was also observed that following pulverization of the larvae, the bacterial abundance increased and the rate of release of bacteria was dependent on power level and treatment time, which in turn decided the pulverization rate and hence the rate of release of bacteria.

Keywords

Balanus amphitriteultrasonic treatmentbacteriapulverization energyacoustic power densitywater treatment
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 7 , November 2010 , pp. 1475 - 1482
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anand, H., Balasundaram, B., Pandit, A.B. and Harrison, S.T.L. (2007) The effect of chemical pretreatment combined with mechanical disruption on the extent of disruption and release of intracellular protein from E. coli. Biochemical Engineering Journal 35, 166–173.CrossRefGoogle Scholar
Anil, A.C., Venkat, K., Sawant, S.S., Dileepkumar, M., Dhargalkar, V.K., Ramaiah, N., Harkantra, S.N. and Ansari, Z.A. (2002) Marine bio-invasion: concern for ecology and shipping. Current Science 83, 214–218.Google Scholar
Ashokkumar, M., Lee, J., Kentish, S. and Grieser, F. (2007) Bubbles in an acoustic field: an overview. Ultrasonics Sonochemistry 14, 470–475.CrossRefGoogle Scholar
Buchholz, K., Deborah, T., Scott, M. and Emmanuele, F. (1998) Ballast water secondary treatment technology review. Battelle Duxbury Operations.Google Scholar
Carlton, J.J. and Geller, J.B. (1993) Ecological roulette: biological invasions and the global transport of non-indigenous marine organisms. Science 261, 78–82.Google Scholar
Crum, L.A., Mason, T.J., Reisse, J.-L. and Suslick, K.S. (1999) Sonochemistry and sonoluminescence. In NATO ASI Series C, Volume 524. Dordrecht, Netherlands: Kluwer Publishers, pp. 191–204.Google Scholar
Daley, R.J. and Hobbie, J.E. (1975) Direct counts of aquatic bacteria by a modified epifluorescence technique. Limnology and Oceanography 20, 875–882.Google Scholar
Davies, R. (1959) Effects on the use of ultrasound waves for the disruption of microorganisms. Biochimica et Biophysica Acta 33, 481–493.Google Scholar
Desai, D.V. and Anil, A.C. (2004) The impact of food type, temperature and starvation on larval development of Balanus amphitrite Darwin (Cirripedia: Thoracica). Journal of Experimental Marine Biology and Ecology 306, 113–137.CrossRefGoogle Scholar
Doulah, M.S. (1977) Mechanism of disintegration of biological cells in ultrasonic cavitation. Biotechnology and Bioengineering 19, 649–660.Google Scholar
Feliu, J.X., Cubarsi, R. and Villaverde, A. (1998) Optimized release of recombinant protein by ultrasonication of E. coli cells. Biotechnology and Bioengineering 58, 536–540.3.0.CO;2-9>CrossRefGoogle ScholarPubMed
Gavand, M.R., McClintock, J.B., Amsler, C.D., Peters, R.W. and Angus, R.A. (2007) Effects of sonication and advanced chemical oxidants on the unicellular alga Dunaliella tertiolecta and cysts, larvae and adults of the brine shrimp Artemia salina: a prospective treatment to eradicate invasive organisms from ballast water. Marine Pollution Bulletin 54, 1777–1788.CrossRefGoogle Scholar
Gogate, P.R. (2002) Cavitation: an auxiliary technique in wastewater treatment schemes. Advances in Environmental Research 6, 335–358.CrossRefGoogle Scholar
Gollasch, S., Lenz, J., Dammer, M. and Andres, H.G. (2000) Survival of tropical ballast water organisms during a cruise from the Indian Ocean to the North Sea. Journal of Plankton Research 22, 923–937.Google Scholar
Hardy, A. (1956) The open sea. Boston, MA: Houghton Mifflin, 335 pp.Google Scholar
Holm, E.R., Stamper, D.M., Brizzolara, R.A., Barnes, L., Deamer, N. and Burkholder, J.M. (2008) Sonication of bacteria, phytoplankton and zooplankton: Application to treatment of ballast water. Marine Pollution Bulletin 56, 1201–1208.Google Scholar
Jeuniaux, C. (1982) La chitine dans le règne animal. Bulletin de la Société Zoologique de France 107, 233–249.Google Scholar
Jyoti, K.K. and Pandit, A.B. (2004) Effect of cavitation on chemical disinfection efficiency. Water Research 38, 2249–2258.CrossRefGoogle ScholarPubMed
Mahulkar, A.V., Riedel, C., Gogate, P.R., Neis, U. and Pandit, A.B. (2009) Effect of dissolved gas on efficacy of sonochemical reactors for microbial cell disruption: experimental and numerical analysis. Ultrasonics Sonochemistry 16, 635–643.Google Scholar
Mason, T.J. (1999) Advances in sonochemistry.Volume 5. Greenwich, USA and London, UK: JAI Press.Google Scholar
Mesbahi, E. (2004) Latest results from testing seven different technologies under the EU MARTOB project—where do we stand now? In Matheickal, J.T. and Raaymakers, S (eds) Globallast Monograph Series No. 15. London: International Maritime Organization, pp. 210–230.Google Scholar
NRC (National Research Council Committee on Ships' Ballast Operations) (1996) Stemming the tide: controlling introduction of non-indigenous species by ships' ballast water. Committee on Ships' Ballast Water. Washington, DC: Marine Board, Commission on Engineering and Technical Systems, National Research Council, 141 pp.Google Scholar
Pennington, J.T. and Hadfield, M.G. (1989) A simple nontoxic method for the decalcification of living invertebrate larvae. Journal of Experimental Marine Biology and Ecology 130, 1–7.CrossRefGoogle Scholar
Price, G.J. (1992) Current trends in sonochemistry. Cambridge, UK: Royal Society of Chemistry.Google Scholar
Raabe, D., Sachs, C. and Romano, P. (2005) The crustacean exoskeleton as an example of a structurally and mechanically graded biological nanocomposite material. Acta Materialia 53, 4281–4292.Google Scholar
Ruiz, G.M., Rawlings, T.K., Dobbs, F.C., Drake, L.A., Mullady, T., Huq, A. and Colwell, R.R. (2000) Global spread of microorganisms by ships—ballast water discharged from vessels harbors a cocktail of potential pathogens. Nature 408, 49–50.Google Scholar
Sawant, S.S., Anil, A.C., Venkat, K., Gaonkar, C., Kolwalkar, J., Khandeparker, L., Desai, D., Mahulkar, A.V., Ranade, V.V. and Pandit, A.B. (2008) Effect of hydrodynamic cavitation on zooplankton: a tool for disinfection. Biochemical Engineering Journal 42, 320–328.Google Scholar
Scherba, G., Weigel, R.M. and O'Brien, Jr W.D. (1991) Quantitative assessment of the germicidal efficacy of ultrasonic energy. Applied and Environmental Microbiology 57, 2079–2084.CrossRefGoogle ScholarPubMed
Sokal, R.R. and Rohlf, F.J. (1981) Biometry. 2nd edition. San Francisco, CA: W.H. Freeman & Company.Google Scholar
Suslick, K.S., Hammerton, D.A. and Cline, R.E. (1986) Sonochemical hot spot. Journal of the American Chemical Society 108, 5641–5642.CrossRefGoogle Scholar
Tiehm, A. (2001) Combination of ultrasonic and biological pollutant degradation. Advances in Sonochemistry 6, 25–58.CrossRefGoogle Scholar
Truby, E.W. (1997) Preparation of single-celled marine dinoflagellates for electron microscopy. Microscopy Research and Technique 36, 337–340.3.0.CO;2-Q>CrossRefGoogle ScholarPubMed
Willis, J.H. (1999) Cuticular proteins in insects and crustaceans. American Zoologist 39, 600–609.CrossRefGoogle Scholar
Young, F.R. (1999) Cavitation. London: Imperial College Press.Google Scholar