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A member of the HSP90 family from ovine Babesia in China: molecular characterization, phylogenetic analysis and antigenicity

Published online by Cambridge University Press:  09 July 2015

GUIQUAN GUAN ,
JUNLONG LIU ,
AIHONG LIU ,
YOUQUAN LI ,
QINGLI NIU ,
JINLIANG GAO ,
JIANXUN LUO ,
ALAIN CHAUVIN ,
HONG YIN  and
EMMANUELLE MOREAU
GUIQUAN GUAN
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China LUNAM Université, Oniris, Ecole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique, UMR Biologie, Epidémiologie et Analyse de Risque en santé animale, CS 40706, F-44307 Nantes, France INRA, UMR1300, F-44307 Nantes, France
JUNLONG LIU
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
AIHONG LIU
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
YOUQUAN LI
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
QINGLI NIU
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
JINLIANG GAO
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
JIANXUN LUO
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China
ALAIN CHAUVIN
Affiliation:
LUNAM Université, Oniris, Ecole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique, UMR Biologie, Epidémiologie et Analyse de Risque en santé animale, CS 40706, F-44307 Nantes, France INRA, UMR1300, F-44307 Nantes, France
HONG YIN*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, People's Republic of China
EMMANUELLE MOREAU*
Affiliation:
LUNAM Université, Oniris, Ecole nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique, UMR Biologie, Epidémiologie et Analyse de Risque en santé animale, CS 40706, F-44307 Nantes, France INRA, UMR1300, F-44307 Nantes, France
*
*Corresponding authors. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China; LUNAM Université, Oniris – Site de la Chantrerie, UMR 1300 BioEpAR, Route de Gachet, BP 40706, F-44307 Nantes Cedex 03, France. E-mail: yinhong@caas.cn; emmanuelle.moreau@oniris-nantes.fr
*Corresponding authors. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, People's Republic of China; LUNAM Université, Oniris – Site de la Chantrerie, UMR 1300 BioEpAR, Route de Gachet, BP 40706, F-44307 Nantes Cedex 03, France. E-mail: yinhong@caas.cn; emmanuelle.moreau@oniris-nantes.fr
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Summary

Heat shock protein 90 (HSP90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. A Hsp90 gene (BQHsp90) was cloned and characterized from Babesia sp. BQ1 (Lintan), an ovine Babesia isolate belonging to Babesia motasi-like group, by screening a cDNA expression library and performing rapid amplification of cDNA ends. The full-length cDNA of BQHsp90 is 2399 bp with an open reading frame of 2154 bp encoding a predicted 83 kDa polypeptide with 717 amino acid residues. It shows significant homology and similar structural characteristics to Hsp90 of other apicomplex organisms. Phylogenetic analysis, based on the HSP90 amino acid sequences, showed that the Babesia genus is clearly separated from other apicomplexa genera. Five Chinese ovine Babesia isolates were divided into 2 phylogenetic clusters, namely Babesia sp. Xinjiang (previously designated a new species) cluster and B. motasi-like cluster which could be further divided into 2 subclusters (Babesia sp. BQ1 (Lintan)/Babesia sp. Tianzhu and Babesia sp. BQ1 (Ningxian)/Babesia sp. Hebei). Finally, the antigenicity of rBQHSP90 protein from prokaryotic expression was also evaluated using western blot and enzyme-linked immunosorbent assay (ELISA).

Keywords

Babesia motasiBabesia sp. BQ1 (Lintan)HSP90phylogenetic analysisantigenicity
Type
Research Article
Information
Parasitology , Volume 142 , Issue 11 , September 2015 , pp. 1387 - 1397
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Copyright
Copyright © Cambridge University Press 2015 

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