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Correction factors for 13C-labelled substrate oxidation at whole-body and muscle level

Published online by Cambridge University Press:  12 June 2007

Gerrit van Hall
Gerrit van Hall*
Affiliation:
The Copenhagen Muscle Research Centre, Rigshospitalet section 7652, 20 Tagensvej, DK-2200, Copenhagen N, Denmark
*
Corresponding Author: Dr G. van Hall, fax +45 3545 7634, email RH01769@RH.DK
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Abstract

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The oxidation of fatty acids, carbohydrates and amino acids can be measured by quantifying the rate of excretion of labelled CO2 following administration of 14C- or 13C-labelled substrates at whole-body and tissue level. However, there is a theoretical need to correct the oxidation rates for the proportion of labelled CO2 that is produced via oxidation but not excreted. Furthermore, depending on the substrate and position of the C label(s), there may also be a need to correct for labelled C from the metabolized substrate that does not appear as CO2, but rather becomes temporarily fixed in other metabolites. The bicarbonate correction factor is used to correct for the labelled CO2 not excreted. Recently, an acetate correction factor has been proposed for the simultaneous correction of CO2 not excreted and label fixed in other metabolites via isotopic exchange reactions, mainly in the tricarboxylic acid cycle. Changes in metabolic rate induced, for example, by feeding, hormonal changes and physical activity, as well as infusion time, have been shown to affect both correction factors. The present paper explains the theoretical and physiological basis of these correction factors and makes recommendations as to how these correction factors should be used in various physiological conditions.

Keywords

BicarbonateAcetateFatty acidsCarbohydrateExercise
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 58 , Issue 4 , November 1999 , pp. 979 - 986
Copyright
The Nutrition Society

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