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Primary structure of arabinoxylans of ispaghula husk and wheat bran.

Published online by Cambridge University Press:  05 March 2007

Sandra Edwards ,
Martin F. Chaplin ,
Anne D. Blackwood  and
Peter W. Dettmar
Sandra Edwards
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Martin F. Chaplin*
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Anne D. Blackwood
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Peter W. Dettmar
Affiliation:
ReckittBenckiser Healthcare (UK) Ltd, Dansom Lane, Kingston upon Hull, HU8 7DS, UK
*
*Corresponding author: Dr Martin Chaplin, fax +44 20 7815 7999, martin.chaplin@sbu.ac.uk
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Abstract

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he primary structures of ispaghula husk and wheat bran were investigated in order to determine how and why these fibres are among the most beneficial dietary fibres. To this end, the polysaccharide preparations have been subjected to enzymic hydrolysis and methylation analysis. The results have shown ispaghula husk and wheat bran to be very-highly-branched arabinoxylans consisting of linear Β-D-→l(4)-linked xylopyranose →Xylp) backbones to which a-L-arabinofuranose →Araf) units are attached as side residues via α-→1(3) and a-→l(2) linkages. Other substituents identified as present in wheat bran include Β-D-glucuronic acid attached via the C→O)-2 position, and arabinose oligomers, consisting of two or more arabinofuranosyl residues linked via 1–2, 1–3, and 1–4 linkages. Ispaghula-husk arabinoxylan is more complex having additional side residues which include a-D-glucuronopyranose →GalAp)-→l→2(-linked-α-L-rhamnopyranose-(1→4)-β-D-Xylp, α-D-GalA/>-→l→3(-linked-a-L-Araf-→1)4(-p-D-Xylp, and <x-L-Araf-→1→3(-linked-p-D-Xylp-→1→4(-β-D-Xylp. The beneficial effects of increased faecal bulk and water-holding capacity are undoubtedly related to the structures of the arabinoxylans, with differences in their efficacy to treat various functional bowel disorders due to their specific structural features

Keywords

Dietary fibreArabinoxylanIspaghula huskWheat bran
Type
Session: Physiological aspects of fibre
Information
Proceedings of the Nutrition Society , Volume 62 , Issue 1 , February 2003 , pp. 217 - 222
Copyright
Copyright © The Nutrition Society 2003

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