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The production of very-long-chain PUFA biosynthesis in transgenic plants: towards a sustainable source of fish oils

Published online by Cambridge University Press:  07 March 2007

Johnathan A. Napier  and
Olga Sayanova
Johnathan A. Napier*
Affiliation:
Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
Olga Sayanova
Affiliation:
Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
*
*Corresponding author: Professor Johnathan Napier, fax +44 1582 763010, johnathan.napier@bbsrc.ac.uk
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Abstract

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There is now considerable evidence of the importance of n-3 long-chain PUFA in human health and development. At the same time, the marine fish stocks that serve as the primary sources of these fatty acids are threatened by continued over-exploitation. Thus, there is an urgent need to provide a sustainable alternative source of the n-3 long-chain PUFA normally found in fish oils. The possibility of using transgenic plants genetically engineered to synthesise these important fatty acids has recently been demonstrated. The approaches taken to realise this outcome will be discussed, as will their prospects for providing a sustainable resource for the future.

Keywords

DesaturaseElongasePUFATransgenic plantsSustainability
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 64 , Issue 3 , August 2005 , pp. 387 - 393
Copyright
Copyright © The Nutrition Society 2005

References

Abbadi, A, Domergue, F, Fahl, A, Ott, C, Bauer, J, Napier, JA, Welti, R, Cirpus, P & Heinz, E (2004) Biosynthesis of very long chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16, 2734–2748.Google Scholar
Abbadi, A, Domergue, F, Meyer, A, Riedel, K, Sperling, P, Zank, T & Heinz, E (2001) Transgenic oilseeds as sustainable source of nutritionally relevant C20 and C22 polyunsaturated fatty acids. European Journal of Lipid Science and Technology 103,106–113.Google Scholar
Bang, HO, Dyerberg, J & Hjoorne, N (1976) The composition of food consumed by Greenland Eskimos. Acta Medica Scandinavica 200, 69–73.CrossRefGoogle ScholarPubMed
Beaudoin, F, Michaelson, LV, Hey, SJ, Lewis, MJ, Shewry, PR, Sayanova, O & Napier, JA (2000) Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway. Proceedings of the National Academy of Sciences USA 97, 6421–6426.Google Scholar
Beaudoin, F & Napier, JA (2004) Biosynthesis and compartmentalisation of triacyglycerol in higher plants. Topics in Current Genetics 6, 267–287.Google Scholar
Berger, A, Monnard, I, Baur, M, Charbonnet, C, Safonova, I & Jomard, A (2002) Epidermal anti-inflammatory properties of 5, 11, 14, 20:3: Effects on mouse ear edema, PGE2 levels in cultured keratinocytes, and PPAR activation. Lipids in Health and Disease 1, 1–12.Google Scholar
Burr, ML, Fehily, AM, Gilbert, JF, Rogers, S, Holliday, RM, Sweetnam, PM, Elwood, PC & Deadman, NM (1989) Effects of changes in fat, fish, and fibre intakes on death and myocardial reinfarction: diet and reinfarction trial (DART). Lancet ii, 757–761.Google Scholar
Domergue, F, Abbadi, A, Ott, C, Zank, TK, Zahringer, U & Heinz, E (2003) Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. Journal of Biological Chemistry 278, 35115–35126.CrossRefGoogle ScholarPubMed
Domergue, F, Lerchl, J, Zahringer, U & Heinz, E (2002) Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis. European Journal of Biochemistry 269, 4105–4113.CrossRefGoogle ScholarPubMed
Drexler, H, Spiekermann, P, Meyer, A, Domergue, F, Zank, T, Sperling, P, Abbadi, A & Heinz, E (2003) Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. Journal of Plant Physiology 160, 779–802.CrossRefGoogle ScholarPubMed
Dyerberg, J & Bang, HO (1982) A hypothesis on the development of acute myocardial infarction in Greenlanders. Scandinavian Journal of Clinical and Laboratory Investigation 161, Suppl. 7–13 Google Scholar
German, JB, Roberts, MA & Watkins, SM (2003) Genomics and metabolomics as markers for the interaction of diet and health: lessons from lipids. Journal of Nutrition 133, 2078S–2083S.Google Scholar
Gil, A, Ramirez, M & Gil, M (2003) Role of long-chain polyunsaturated fatty acids in infant nutrition. European Journal of Clinical Nutrition 57, Suppl. 1. S31–S34 Google Scholar
GISSI-Prevenzione Investigators (1999) Dietary supplementation with n -3 polyunsaturated fatty acids and vitamin E after myocardial infarction: results of the GISSI-Prevenzione trial. Gruppo Italiano per lo Studio della Sopravvivenza nell'Infarto miocardico. Lancet 354, 447–455.Google Scholar
Go, VL, Butrum, RR & Wong, DA (2003) Diet, nutrition, and cancer prevention: the postgenomic era. Journal of Nutrition 133, 3830S–3836S.Google Scholar
Graham, IA, Cirpus, P, Rein, D & Napier, JA (2004) The use of very long chain polyunsaturated fatty acids to ameliorate Metabolic Syndrome: transgenic plants as an alternative sustainable source to fish oils. Nutrition Bulletin 29, 228–233.Google Scholar
Green, AG (2004) From alpha to omega-producing essential fatty acids in plants. Nature Biotechnology 22, 680–682.CrossRefGoogle ScholarPubMed
Hu, FB, Manson, JE & Willett, WC (2001) Types of dietary fat and risk of coronary heart disease: a critical review. Journal of the American College of Nutrition 20, 5–19.Google Scholar
Jaworski, J & Cahoon, EB (2003) Industrial oils from transgenic plants. Current Opinion in Plant Biology 6, 178–184.Google Scholar
Klaver, CC & Allikmets, R (2003) Genetics of macular dystrophies and implications for age-related macular degeneration. Developments in Ophthalmology 37, 155–169.Google Scholar
Knutzon, DS, Thurmond, JM, Huang, YS, Chaudhary, S, Bobik EG, Jr, Chan, GM, Kirchner, SM, Mukerji, P (1998) Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canola. Journal of Biological Chemistry 273, 29360–29366.Google Scholar
Leonard, AE, Pereira, SL, Sprecher, H & Huang, YS (2004) Elongation of long-chain fatty acids. Progress in Lipid Research 43, 36–54.Google Scholar
Metz, JG, Roessler, P, Facciotti, D, Levering, C, Dittrich, F, Lassner, M et al. (2001) Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293, 290–293.Google Scholar
Meyer, A, Kirsch, H, Domergue, F, Abbadi, A, Sperling, P, Bauer, J et al. (2004) Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. Journal of Lipid Research 45, 1899–909.CrossRefGoogle ScholarPubMed
Millar, AA, Wrischer, M & Kunst, L (1998) Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology. Plant Cell 10, 1889–1902.Google Scholar
Nakane, S, Tanaka, T, Satouchi, K, Kobayashi, Y, Waku, K & Sugiura, T (2000) Occurrence of a novel cannabimimetic molecule 2-sciadonoylglycerol (2-eicosa-5', 11', 14'-trienoylglycerol) in the umbrella pine Sciadopitys verticillata seeds. Biological and Pharmaceutical Bulletin 23, 758–761.Google Scholar
Napier, JA (2002) Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms. Trends in Plant Science 7, 51–54.Google Scholar
Napier, JA, Michaelson, LV & Sayanova, O (2003) The role of cytochrome b 5 fusion desaturases in the synthesis of polyunsaturated fatty acids. Prostaglandins, Leukotrienes, and Essential Fatty Acids 68, 135–143.Google Scholar
Napier, JA, Sayanova, O, Qi, B & Lazarus, CM (2004) Progress towards the production of polyunsaturated fatty acids in transgenic plants. Lipids 39, 1067–1075.Google Scholar
Nugent, AP (2004) The metabolic syndrome. Nutrition Bulletin 29, 36–43.Google Scholar
Opsahl-Ferstad, H-G, Rudi, H, Ruyter, B & Refstie, S (2003) Biotechnological approaches to modify rapeseed oil composition for applications in aquaculture. Plant Science 165, 349–357.CrossRefGoogle Scholar
Paoloni-Giacobino, A, Grimble, R & Pichard, C (2003) Genetics and nutrition. Clinical Nutrition 22, 429–435.CrossRefGoogle ScholarPubMed
Parker-Barnes, JM, Das, T, Bobik, E, Leonard, AE, Thurmond, JM, Chaung, LT, Huang, YS & Mukerji, P (2000) Identification and characterization of an enzyme involved in the elongation of n -6 and n -3 polyunsaturated fatty acids. Proceedings of the National Academy of Sciences USA 97, 8284–8289.Google Scholar
Pereira, SL, Leonard, AE, Huang, YS, Chuang, LT & Mukerji, P (2004) Identification of two novel microalgal enzymes involved in the conversion of the omega 3-fatty acid, eicosapentaenoic acid (EPA), to docosahexaenoic acid (DHA). Biochemical Journal 384, 357–366.Google Scholar
Qi, B, Beaudoin, F, Fraser, T, Stobart, AK, Napier, JA & Lazarus, CM (2002) Identification of a cDNA encoding a novel C 18 -Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS Letters 510, 159–165.Google Scholar
Qi, B, Fraser, T, Mugford, S, Dobson, G, Sayanova, O, Butler, J, Napier, JA, Stobart, AK & Lazarus, CM (2004) The production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in transgenic plants. Nature Biotechnology 22, 739–745.Google Scholar
Qiu, X (2003) Biosynthesis of docosahexaenoic acid (DHA, 22:6–4. 7, 10, 13, 16, 19): two distinct pathways. Prostaglandins, Leukotrienes, and Essential Fatty Acids 68, 181–186.CrossRefGoogle Scholar
Qiu, X, Hong, H & MacKenzie, SL (2001) Identification of a Δ4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea. Journal of Biological Chemistry 276, 31561–31566.Google Scholar
Sargent, JR & Tacon, AG (1999) Development of farmed fish: a nutritionally necessary alternative to meat. Proceedings of the Nutrition Society 58, 377–383.Google Scholar
Sayanova, O & Napier, JA (2004) Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry 65, 147–158.Google Scholar
Shastry, BS (2002) SNP alleles in human disease and evolution. Journal of Human Genetics 47, 561–566.Google Scholar
Simopoulos, AP (1999) Essential fatty acids in health and chronic disease. American Journal of Clinical Nutrition 70, 560S–569S.Google Scholar
Simopoulos, AP (2000) Human requirement for n -3 polyunsaturated fatty acids. Poultry Science 79, 961–970.Google Scholar
Sperling, P, Ternes, P, Zank, TK & Heinz, E (2003) The evolution of desaturases. Prostaglandins, Leukotrienes, and Essential Fatty Acids 68, 73–95.Google Scholar
Thelen, JJ & Ohlrogge, JB (2002) Metabolic engineering of fatty acid biosynthesis in plants. Metabolic Engineering 4, 12–21.Google Scholar
Tocher, DR & Ghioni, C (1999) Fatty acid metabolism in marine fish: low activity of fatty acyl Δ5 desaturation in gilthead sea bream ( Sparus aurata ) cells. Lipids 34, 433–440.Google Scholar
Tucker, G (2003) Nutritional enhancement of plants. Current Opinion in Biotechnology 14, 221–225.Google Scholar
von Schacky, C (2003) The role of omega-3 fatty acids in cardiovascular disease. Current Atherosclerosis Reports 5, 139–145.Google Scholar
Wallis, JG & Browse, J (1999) The Δ8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. Archives of Biochemistry and Biophysics 365, 307–316.Google Scholar
Wallis, JG, Watts, JL & Browse, J (2002) Polyunsaturated fatty acid synthesis: what will they think of next. Trends in Biochemical Sciences 27, 467–470.CrossRefGoogle ScholarPubMed
Zhao, Z, Fu, YX, Hewett-Emmett, D & Boerwinkle, E (2003) Investigating single nucleotide polymorphism (SNP) density in the human genome and its implications for molecular evolution. Gene 312, 207–213.Google Scholar