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Studies of plasma membrane fatty acid-binding protein and other lipid-binding proteins in human skeletal muscle

Published online by Cambridge University Press:  05 March 2007

C. Roepstorff ,
J. Wulff Helge ,
B. Vistisen  and
B. Kiens
C. Roepstorff
Affiliation:
Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 13 Universitetsparken, 2100, Copenhagen Ø, Denmark
J. Wulff Helge
Affiliation:
Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 13 Universitetsparken, 2100, Copenhagen Ø, Denmark
B. Vistisen
Affiliation:
Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 13 Universitetsparken, 2100, Copenhagen Ø, Denmark
B. Kiens*
Affiliation:
Copenhagen Muscle Research Centre, Department of Human Physiology, Institute of Exercise and Sport Sciences, University of Copenhagen, 13 Universitetsparken, 2100, Copenhagen Ø, Denmark
*
*Corresponding author: Dr Bente Kiens Fax: +45 35 32 1600, Email: bkiens@aki.ku.dk
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Abstract

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The first putative fatty acid transporter identified was plasma membrane fatty acid-binding protein (FABPpm). Later it was demonstrated that this protein is identical to the mitochondrial isoform of the enzyme aspartate aminotransferase. In recent years data from several cell types have emerged, indicating that FABPpm plays a role in the transport of long-chain saturated and unsaturated fatty acids. In the limited number of studies in human skeletal muscle it has been demonstrated that dietary composition and exercise training can influence the content of FABPpm. Ingestion of a fat-rich diet induces an increase in FABPpm protein content in human skeletal muscle in contrast to the decrease seen during consumption of a carbohydrate-rich diet. A similar effect of a fat-rich diet is also observed for cytosolic fatty acid-binding protein and fatty acid translocase/CD36 protein expression. Exercise training up regulates FABPpm protein content in skeletal muscle, but only in male subjects; no significant differences were observed in muscle FABPpm content in a cross-sectional study of female volunteers of varying training status, even though muscle FABPpm content did not depend on gender in the untrained state. A higher utilization of plasma long-chain fatty acids during exercise in males compared with females could explain the gender-dependent influence of exercise training on FABPpm. The mechanisms involved in the regulation of the function and expression of FABPpm protein remain to be clarified.

Keywords

DietGenderExerciseTrainingLipid-binding proteins
Type
Symposium 2: The fatty acid transporters of skeletal muscle
Information
Proceedings of the Nutrition Society , Volume 63 , Issue 2 , May 2004 , pp. 239 - 244
Copyright
Copyright © The Nutrition Society 2004

References

Abumrad, N, Coburn, C & Ibrahimi, A (1999) Membrane proteins implicated in long-chain fatty acid uptake by mammalian cells: CD36, FATP and FABPm. Biochimica et Biophysica Acta 1441, 4–13.CrossRefGoogle ScholarPubMed
Abumrad, NA, el Maghrabi, MR, Amri, EZ, Lopez, E & Grimaldi, PA (1993) Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation. Homology with human CD36. Journal of Biological Chemistry 268, 17665–17668.Google Scholar
Berk, PD & Stump, DD (1999) Mechanisms of cellular uptake of long chain free fatty acids. Molecular and Cellular Biochemistry 192, 17–31.Google Scholar
Berk, PD, Wada, H, Horio, Y, Potter, BJ, Sorrentino, D, Zhou, SL, Isola, LM, Stump, D, Kiang, CL & Thung, S (1990) Plasma membrane fatty acid-binding protein and mitochondrial glutamic-oxaloacetic transaminase of rat liver are related. Proceedings of the National Academy of Sciences USA 87, 3484–3488.Google Scholar
Besnard, P, Niot, I, Poirier, H, Clement, L & Bernard, A (2002) New insights into the fatty acid-binding protein (FABP) family in the small intestine. Molecular and Cellular Biochemistry 239, 139–147.Google Scholar
Bonen, A, Dyck, DJ & Luiken, JJ (1998a) Skeletal muscle fatty acid transport and transporters. Advances in Experimental Medicine and Biology 441, 193–205.Google Scholar
Bonen, A, Luiken, JJ, Liu, S, Dyck, DJ, Kiens, B, Kristiansen, S, Turcotte, LP, van der Vusse, GJ & Glatz, JF (1998b) Palmitate transport and fatty acid transporters in red and white muscles. American Journal of Physiology 275, E471–E478.Google ScholarPubMed
Bonen, A, Miskovic, D & Kiens, B (1999) Fatty acid transporters (FABPpm, FAT, FATP) in human muscle. Canadian Journal of Applied Physiology 24, 515–523.Google Scholar
Bradbury, MW & Berk, PD (2000) Mitochondrial aspartate aminotransferase: direction of a single protein with two distinct functions to two subcellular sites does not require alternative splicing of the mRNA. Biochemical Journal 345, 423–427.CrossRefGoogle Scholar
Cameron-Smith, D, Burke, LM, Angus, DJ, Tunstall, RJ, Cox, GR, Bonen, A, Hawley, JA & Hargreaves, M (2003) A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle. American Journal of Clinical Nutrition 77, 313–318.Google Scholar
Cechetto, JD, Sadacharan, SK, Berk, PD & Gupta, RS (2002) Immunogold localization of mitochondrial aspartate aminotransferase in mitochondria and on the cell surface in normal rat tissues. Histology and Histopathology 17, 353–364.Google Scholar
Clavel, S, Farout, L, Briand, M, Briand, Y & Jouanel, P (2002) Effect of endurance training and/or fish oil supplemented diet on cytoplasmic fatty acid binding protein in rat skeletal muscles and heart. European Journal of Applied Physiology 87, 193–201.CrossRefGoogle ScholarPubMed
Coe, NR & Bernlohr, DA (1998) Physiological properties and functions of intracellular fatty acid-binding proteins. Biochimica et Biophysica Acta 1391, 287–306.Google Scholar
Faergeman, NJ & Knudsen, J (1997) Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling. Biochemical Journal 323, 1–12.CrossRefGoogle ScholarPubMed
Glatz, JF & Storch, J (2001) Unravelling the significance of cellular fatty acid-binding proteins. Current Opinion in Lipidology 12, 267–274.Google Scholar
Glatz, JF, Vork, MM, Cistola, DP & van der Vusse, GJ (1993) Cytoplasmic fatty acid binding protein: significance for intracellular transport of fatty acids and putative role on signal transduction pathways. Prostaglandins, Leukotrienes and Essential Fatty Acids 48, 33–41.Google Scholar
Grimaldi, PA, Teboul, L, Gaillard, D, Armengod, AV & Amri, EZ (1999) Long chain fatty acids as modulators of gene transcription in preadipose cells. Molecular and Cellular Biochemistry 192, 63–68.CrossRefGoogle ScholarPubMed
Helge, JW & Kiens, B (1997) Muscle enzyme activity in humans: role of substrate availability and training. American Journal of Physiology 272, R1620–R1624.Google ScholarPubMed
Helge, JW, Richter, EA & Kiens, B (1996) Interaction of training and diet on metabolism and endurance during exercise in man. Journal of Physiology (London) 492, 293–306.Google Scholar
Helge, JW, Watt, PW, Richter, EA, Rennie, MJ & Kiens, B (2001) Fat utilization during exercise: adaptation to a fat-rich diet increases utilization of plasma fatty acids and very low density lipoprotein-triacylglycerol in humans. Journal of Physiology (London) 537, 1009–1020.Google Scholar
Helge, JW, Wulff, B & Kiens, B (1998) Impact of a fat-rich diet on endurance in man: role of the dietary period. Medicine and Science in Sports and Exercise 30, 456–461.Google Scholar
Hirsch, D, Stahl, A & Lodish, HF (1998) A family of fatty acid transporters conserved from mycobacterium to man. Proceedings of the National Academy of Sciences USA 95, 8625–8629.Google Scholar
Kiens, B, Essen-Gustavsson, B, Christensen, NJ & Saltin, B (1993) Skeletal muscle substrate utilization during submaximal exercise in man: effect of endurance training. Journal of Physiology (London) 469, 459–478.CrossRefGoogle ScholarPubMed
Kiens, B, Essen-Gustavsson, B, Gad, P & Lithell, H (1987) Lipoprotein lipase activity and intramuscular triglyceride stores after long-term high-fat and high-carbohydrate diets in physically trained men. Clinical Physiology 7, 1–9.Google Scholar
Kiens, B, Kristiansen, S, Jensen, P, Richter, EA & Turcotte, LP (1997) Membrane associated fatty acid binding protein (FABPpm) in human skeletal muscle is increased by endurance training. Biochemical and Biophysical Research Communications 231, 463–465.Google Scholar
Luiken, JJ, Turcotte, LP & Bonen, A (1999) Protein-mediated palmitate uptake and expression of fatty acid transport proteins in heart giant vesicles. Journal of Lipid Research 40, 1007–1016.Google Scholar
Mogensen, IB, Schulenberg, H, Hansen, HO, Spener, F & Knudsen, J (1987) A novel acyl-CoA-binding protein from bovine liver. Effect on fatty acid synthesis. Biochemical Journal 241, 189–192.CrossRefGoogle ScholarPubMed
Motojima, K, Passilly, P, Peters, JM, Gonzalez, FJ & Latruffe, N (1998) Expression of putative fatty acid transporter genes are regulated by peroxisome proliferator-activated receptor alpha and gamma activators in a tissue- and inducer-specific manner. Journal of Biological Chemistry 273, 16710–16714.CrossRefGoogle Scholar
Roepstorff, C, Steffensen, CH, Madsen, M, Stallknecht, B, Kanstrup, IL, Richter, EA & Kiens, B (2002) Gender differences in substrate utilization during submaximal exercise in endurance-trained subjects. American Journal of Physiology 282, E435–E447.Google ScholarPubMed
Schaffer, JE & Lodish, HF (1994) Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell 79, 427–436.CrossRefGoogle ScholarPubMed
Schwieterman, W, Sorrentino, D, Potter, BJ, Rand, J, Kiang, CL, Stump, D & Berk, PD (1988) Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut. Proceedings of the National Academy of Sciences USA 85, 359–363.CrossRefGoogle ScholarPubMed
Sorrentino, D, Stump, D, Robinson, RB, White, R, Kiang, CL & Berk, PD (1988) Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut. Journal of Clinical Investigation 82, 928–935.Google Scholar
Steffensen, CH, Roepstorff, C, Madsen, M & Kiens, B (2002) Myocellular triacylglycerol breakdown in females but not in males during exercise. American Journal of Physiology 282, E634–E642.Google Scholar
Storch, J & Thumser, AE (2000) The fatty acid transport function of fatty acid-binding proteins. Biochimica et Biophysica Acta 1486, 28–44.Google Scholar
Stremmel, W (1988) Uptake of fatty acids by jejunal mucosal cells is mediated by a fatty acid binding membrane protein. Journal of Clinical Investigation 82, 2001–2010.Google Scholar
Stremmel, W, Strohmeyer, G, Borchard, F, Kochwa, S & Berk, PD (1985) Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes. Proceedings of the National Academy of Sciences USA 82, 4–8.Google Scholar
Stump, DD, Zhou, SL & Berk, PD (1993) Comparison of plasma membrane FABP and mitochondrial isoform of aspartate aminotransferase from rat liver. American Journal of Physiology 265, G894–G902.Google ScholarPubMed
Turcotte, LP (1999) Fatty acid binding proteins and muscle lipid metabolism in skeletal muscle. In Biochemistry of Exercise, vol. 5 pp. 201–215 [Hargreaves, M, editor]. Champaign, IL: Human Kinetics.Google Scholar
Turcotte, LP, Swenberger, JR, Tucker, MZ & Yee, AJ (1999) Training-induced elevation in FABPPM is associated with increased palmitate use in contracting muscle. Journal of Applied Physiology 87, 285–293.Google Scholar
Van Bilsen, M, van der Vusse, GJ, Gilde, AJ, Lindhout, M & Van der Lee, KA (2002) Peroxisome proliferator-activated receptors: lipid binding proteins controlling gene expression. Molecular and Cellular Biochemistry 239, 131–138.Google Scholar
Wolfrum, C, Borrmann, CM, Borchers, T & Spener, F (2001) Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors alpha- and gamma-mediated gene expression via liver fatty acid binding protein: a signaling path to the nucleus. Proceedings of the National Academy of Sciences USA 98, 2323–2328.Google Scholar
Xu, HE, Lambert, MH, Montana, VG, Parks, DJ, Blanchard, SG, Brown, PJ, Sternbach, DD, Lehmann, JM, Wisely, GB, Willson, TM, Kliewer, SA & Milburn, MV (1999) Molecular recognition of fatty acids by peroxisome proliferator-activated receptors. Molecular Cell 3, 397–403.CrossRefGoogle ScholarPubMed
Zhou, SL, Stump, D, Sorrentino, D, Potter, BJ & Berk, PD (1992) Adipocyte differentiation of 3T3-L1 cells involves augmented expression of a 43-kDa plasma membrane fatty acid-binding protein. Journal of Biological Chemistry 267, 14456–14461.Google Scholar